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Expression of Lupinus albus PR-10 proteins during root and leaf development
Pinto, Paula; Ribeiro, A.; Regalado, A. P.; Rodrigues-Pousada, C.; Ricardo, C. P. P.Based on the NH2-terminal sequence of three PR-10 isoforms previously identified in Lupinus albus leaves and a conserved amino-acid region in the PR-10 proteins from leguminosae, a pair of oligonucleotides was designed and used to amplify the corresponding cDNA fragment from a L. albus leaves cDNA library. A fragment of DNA of 200 bp was isolated from the polymerase chain reaction (PCR) mixture and subsequently...
Lupinus albus L. pathogenesis-related proteins that show similarity to PR-10 pr...
Pinto, Paula; Ricardo, C. P. P.We describe a group of three acidic proteins, pathogenesis-related (PR)-p16.5a, PR-p16.5b, and PR-p16.5c, that accumulate in the leaves of Lupinus albus L. cv Rio Maior plants when infected with the fungus Colletotrichum gloeosporioides Penz. These proteins co-migrate in sodium dodecyl sulfate-polyacrylamide gels as a single band of 16.5 kD, behaving as charge isomers, and are related to several members of the ...
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