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In vitro and computational studies of transdermal perfusion of nanoformulations...

Transdermal perfusion of a large protein is reported for the first time, using a nanoemulsion of bovine serum albumin (66 kDa) of 160 nm prepared by a solid-in-oil (S/O) process. Molecular dynamics simulations confirmed skin permeation by these formulations, with integration of the protein into the lipid bilayers. These results demonstrate the real possibility of delivering large proteins transdermally for a ra...

Nanostructured thin coatings from chitosan and an elastin-like recombinamer wit...

Publicado em: Materials Science Forum, vols. 730-732; Online available since 2012/Nov/12 ; In the present work, chitosan (CHI) and elastin-like recombinamers (ELRs) were used to conceive nanostructured thin films driven by sequential electrostatic layer-by-layer (LbL), a simple and versatile technique that discards the use of harmful reagents. Two similar ELRs were engineered to contain negatively charged amin...

Potential of human γD-crystallin for hair damage repair : insights into the mec...

The objective of this work was to develop a new strategy to physically ‘repair’ chemically damaged hair. Hence the human eye γD-crystallin, a protein from the superfamily characterized structurally by the Greek key motif, was studied. The human γD-crystallin was chosen based on the ability of proteins belonging to this superfamily to be involved in the coating of specific structures. Two crystallins were used o...

A synthetic biology approach for the development of recombinant protein-based p...

The search for advanced materials, especially for those bioinspired, has recently been the focus of great research in many fields of application. Due to recent advances in materials characterization and fabrication technologies, and especially through the use of synthetic biology approaches, it is now possible to reengineer novel functionalities and structures of protein-based materials, taking advantage ...

Antibacterial and antifungal activity of a new solvent-cast film based on a fun...

Developing scaffolds for tissue engineering using the Ca2+-induced cold gelatio...

The Ca2+-induced cold gelation technique was found suitable to prepare highly porous biodegradable scaffolds based on bovine serum albumin (BSA) and alpha-casein from bovine milk for tissue engineering. A 23 full factorial design was used to study the influence and impact of each factor on the several responses of the scaffolds. In vitro degradation (ID), swelling ratio (SR), porosity (PO), and pore size (PS) a...

Exploiting the sequence of naturally occurring elastin : construction, producti...

Genetic engineering was used to produce an elastin-like polymer (ELP) with precise amino acid composition, sequence and length, resulting in the absolute control of MW and stereochemistry. A synthetic monomer DNA sequence encoding for (VPAVG)20, was used to build a library of concatemer genes with precise control on sequence and size. The higher molecular weight polymer with 220 repeats of VPAVG was biologi...