Encontrados 5 documentos, a visualizar página 1 de 1
A 32-KDa kilodalton hydrolase plays an important role in Paracoccidioides brasi...
Hernández, Orville; Almeida, A. J.; Gonzalez, Angel; Garcia, Ana Maria; Tamayo, Diana; Cano, L. E.; Restrepo, A.; McEwen, J. G.One of the most crucial events during infection with the dimorphic fungus Paracoccidioides brasiliensis is adhesion to pulmonary epithelial cells, a pivotal step in the establishment of disease. In this study, we have evaluated the relevance of a 32-kDa protein, a putative adhesion member of the haloacid dehalogenase (HAD) superfamily of hydrolases, in the virulence of this fungus. Protein sequence analyses hav...
Genome size and ploidy of Paracoccidioides brasiliensis reveals a haploid DNA c...
Almeida, A. J.; Matute, D. R.; Carmona, J. A.; Martins, M.; Torres, I.; McEwen, J. G.; Restrepo, A.; Leão, Cecília; Ludovico, PaulaThe aim of this study was to evaluate genome size and ploidy of the dimorphic pathogenic fungus Paracoccidioides brasiliensis. The cell cycle analysis of 10 P. brasiliensis isolates by flow cytometry (FCM) revealed a genome size ranging from 26.3+/-0.1Mb (26.9+/-0.1fg) to 35.5+/-0.2Mb (36.3+/-0.2fg) per uninucleated yeast cell. The DNA content of conidia from P. brasiliensis ATCC 60855-30.2+/-0.8Mb (30.9+/-0.8f...
New insights into the cell cycle profile of Paracoccidioides brasiliensis
Almeida, A. J.; Martins, M.; Carmona, J. A.; Cano, L. E.; Restrepo, A.; Leão, Cecília; Rodrigues, Fernando José dos SantosThe present work focuses on the analysis of cell cycle progression of Paracoccidioides brasiliensis yeast cells under diVerent environmental conditions. We optimized a Xow cytometric technique for cell cycle proWle analysis based on high resolution measurements of nuclear DNA. Exponentially growing cells in poor-deWned or rich-complex nutritional environments showed an increased percentage of daughter cells in ...
PCR with Paracoccidioides brasiliensis specific primers: potential use in ecol...
DÍEZ,S.; GARCIA,E.A.; PINO,P.A.; BOTERO,S.; CORREDOR,G.G.; PERALTA,L.A.; CASTAÑO,J.H.; RESTREPO,A.; McEWEN,J.G.The precise microenvironment of Paracoccidioides brasiliensis has not yet been discovered perhaps because the methods used are not sensitive enough. We applied to this purpose the polymerase chain reaction (PCR) using three sets of specific primers corresponding to two P. brasiliensis genes. This fungus as well as several other fungi, were grown and their DNA obtained by mechanical disruption and a phenol chlor...
Inapparent lung involvement in patients with the subacute juvenile type of para...
Restrepo,A.; Trujillo,M.; Gomez,I.Three patients with the diagnosis of subacute juvenile paracoccidioidomycosis who, at the time of their first visit, had no signs or symptoms of lung involvement, were studied. Initially the diagnosis was confirmed by the observation of P. brasiliensis in biopsy material obtained from clinically involved lymphadenopathies. The lung X-rays done in all patients, did not reveal pathologic changes, although it was ...
| Financiadores do RCAAP | |||||||
|
|
|
|
|
|
||