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Integrated microbial processes for biofuels and high value-added products: the ...

The production of microbial biofuels is currently under investigation, as they are alternative sources to fossil fuels, which are diminishing and their use has a negative impact on the environment. However, so far, biofuels derived from microbes are not economically competitive. One way to overcome this bottleneck is the use of microorganisms to transform substrates into biofuels and high value-added products,...

New at-line flow cytometric protocols for determining carotenoidcontent and cel...

Rhodosporidium toruloides NCYC 921 batch growth was monitored as a means to evaluate the yeastbiomass potential as a source for the production of carotenoids and other lipids.Carotenoid content, cell viability and size were assessed by multiparameter flow cytometry. Thesaponifiable lipid fraction was assayed by gas–liquid chromatography.The carotenoid production increased during the stationary phase, reaching 7...

Chemical and biological-based isoprene production: Green metrics

Green metrics is a methodology which allows the greenness of either new or already existing processesto be assessed. This paper is a part of a special issue devoted to green metrics in which this methodologyis applied to different processes to assess bio and petrochemical routes. In this work, green metrics wereused as a tool to validate and compare the petrochemical and biological processes of isoprene product...

Isolamento e seleção de estirpes locais de Haematococcus pluvialis Flotow par...

As microalgas nativas estão bem adaptadas aos fatores abióticos e bióticos locais predominantes e quando cultivadas localmente é fundamental a seleção de estirpes com potencial biotecnológico. Duas estirpes locais de H. pluvialis foram identificadas e isoladas de poças de água doce em locais distintos. Foram cultivadas por um período de 60 dias em meio de cultura MES esterilizado em câmara de cultura...

Biorrefinarias de microalgas

As microalgas autotróficas são organismos fotossintéticos, que graças à sua “maquinaria fotossintética” são capazes de transformar a energia luminosa em energia química com a produção de compostos orgânicos. Os propósitos económicos de produção de biomassa microalgal têm-se alterado ao longo das últimas décadas: após a fase inicial de produção de SCP (single cell protein) para alimentar um mundo carente de alim...

Green metrics evaluation of isoprene production by microalgae and bacteria

Isoprene is a key intermediate compound for the production of synthetic rubber and adhesives and is also used as a building block in the chemical industry. Traditionally, isoprene is obtained from crude oil during the refinery process. Nevertheless, plants and animals are also able to synthesize this important compound. This work compares two renewable approaches for isoprene production: by photosynthetic organ...

Algal biomass production and wastewater treatment in high rate algal ponds rece...

Algal biomass production associated with wastewater is usually carried out in high rate algal ponds (HRAPs), which are concomitantly used in the treatment of such effluent. However, most types of wastewater have high levels of bacteria that can inhibit the growth of algal biomass by competing for space and nutrients. The objective of this study was to assess the influence of ultraviolet (UV) pre-disinfection on...

Enhanced lipidic algae biomass production using gas transfer from a fermentativ...

In order to produce single-cell oil for biodiesel, a yeast and a microalga were, for the first time, grown in two separate reactors connected by their gas-phases, taking advantage of their complementary nutritional metabolisms, i.e., respiration and photosynthesis. The yeast Rhodosporidium toruloides was used for lipid production, originating a carbon dioxideenriched outlet gas stream which in turn was used to ...

Biodiesel from heterotrophic and autotrophic microalgae: a sustainable producti...

Biodiesel from microalgae Chlorella protothecoides growing at autotrophic and h...

Biodiesel is an alternative energy to fossil fuels, and is produced from biomass, therefore provides lower CO2 emissions. Currently, biodiesel is produced from plant oils, animal fats and used oils. These sources are low-yield which is a limitation to the production of biodiesel in large quantities. On the contrary, microalgae present a very high yield and can be produced in large amounts. The bio-refinery of m...