Encontrados 10 documentos, a visualizar página 1 de 1
Revisiting oxidative stress and mitochondrial dysfunction in the pathogenesis o...
Perfeito, Rita; Cunha-Oliveira, Teresa; Rego, Ana CristinaParkinson disease (PD) is a chronic and progressive neurological disease associated with a loss of dopaminergic neurons. In most cases the disease is sporadic but genetically inherited cases also exist. One of the major pathological features of PD is the presence of aggregates that localize in neuronal cytoplasm as Lewy bodies, mainly composed of α-synuclein (α-syn) and ubiquitin. The selective degeneration of ...
Glutathione redox cycle dysregulation in Huntington’s disease knock-in striatal...
Ribeiro, Márcio; Rosenstock, Tatiana; Cunha-Oliveira, Teresa; Ferreira, Ildete; Oliveira, Catarina R.; Rego, Ana CristinaHuntington’s disease (HD) is a CAG repeat disorder affecting the HD gene, which encodes for huntingtin (Htt) and is characterized by prominent cell death in the striatum. Oxidative stress was previously implicated in HD neurodegeneration, but the role of the major endogenous antioxidant system, the glutathione redox cycle, has been less studied following expression of full-length mutant Htt (FL-mHtt). Thus, in ...
Cellular and molecular mechanisms involved in the neurotoxicity of opioid and p...
Cunha-Oliveira, Teresa; Rego, Ana Cristina; Oliveira, Catarina R.Substance abuse and addiction are the most costly of all the neuropsychiatric disorders. In the last decades, much progress has been achieved in understanding the effects of the drugs of abuse in the brain. However, efficient treatments that prevent relapse have not been developed. Drug addiction is now considered a brain disease, because the abuse of drugs affects several brain functions. Neurological impairme...
Mechanisms of neurodegeneration in Huntington's disease
Gil, Joana M.; Rego, Ana CristinaHuntington's disease (HD) is caused by an expansion of cytosine2013adenine2013guanine (CAG) repeats in the huntingtin gene, which leads to neuronal loss in the striatum and cortex and to the appearance of neuronal intranuclear inclusions of mutant huntingtin. Huntingtin plays a role in protein trafficking, vesicle transport, postsynaptic signaling, transcriptional regulation, and apoptosis. Thus, a loss of func...
Adenosine A2AReceptors Regulate the Extracellular Accumulation of Excitatory Am...
Rego, Ana Cristina; Agostinho, Paula; Melo, Joana; Cunha, Rodrigo A.; Oliveira, Catarina R.The role of endogenous extracellular adenosine as a tonic modulator of the extracellular accumulation of excitatory amino acids (glutamate and aspartate) caused by metabolic inhibition was investigated in cultured retinal cells. The selective adenosine A2Areceptor antagonist, 4-[2-[7-amino-2-(2-furyl)(1,2,4)-triazin-5-ylamino]-ethyl]phenol (ZM241385) (50 n ), increased the release of glutamate (three- to four-f...
Influence of the antioxidants vitamin E and idebenone on retinal cell injury me...
Rego, Ana Cristina; Santos, Maria Sancha; Oliveira, Catarina R.A role for the antioxidants vitamin E and idebenone in decreasing retinal cell injury, after metabolic inhibition induced by chemical ischemia and hypoglycemia, was investigated and compared with oxidative stress conditions. Preincubation of the antioxidants, vitamin E (20 [mu]M) and idebenone (10 [mu]M), effectively protected from retinal cell injury after oxidative stress or hypoglycemia, whereas the protecti...
Glutamate-mediated inhibition of oxidative phosphorylation in cultured retinal ...
Rego, Ana Cristina; Sancha Santos, Maria; Oliveira, Catarina ResendeGlutamate is an excitotoxin responsible for causing neuronal damage associated with mitochondria dysfunction. We have analyzed the relationship between the mitochondrial respiratory rate, the membrane potential ([Delta][Psi]) and the activity of mitochondrial complexes in retinal cells in culture, used as neuronal models. Glutamate (10 [mu]M-10 mM) dose-dependently decreased the O2 consumption and the membrane ...
Distinct Glycolysis Inhibitors Determine Retinal Cell Sensitivity to Glutamate-...
Rego, Ana Cristina; Areias, Filipe; Santos, Maria Sancha; Oliveira, CatarinaIn this study, we analyzed how distinct glycolysis inhibitors influenced the redox status of retinal cells, used as a neuronal model. Three different approaches were used to inhibit glycolysis: the cells were submitted to iodoacetic acid (IAA), an inhibitor of glyceraldehyde 3-phosphate dehydrogenase, to 2-deoxy-glucose (DG) in glucose-free medium, which was used as a substitute of glucose, or in the absence of...
Effect of Glucose Deprivation and Acute Glutamate Exposure in Cultured Retinal ...
Rego, Ana Cristina; Areias, Filipe Miguel; Santos, Maria Sancha; Oliveira, Catarina R.The relationship between bioenergetics and the glutamate system was analyzed in a neuronal model of retinal cells in culture, submitted to glucose deprivation and exposed to glutamate for 2 h, and compared with exposure to glutamate in the presence of glucose. Under glucose deprivation, a reduction (about 1.1-fold) in the energy charge of the cells occurred, probably as a result of a decrement (by about 75%) in...
Influence of vitamin E succinate on retinal cell survival
Rego, Ana Cristina; Santos, Maria Sancha; Proença, Maria Teresa; Oliveira, Catarina R.In this study, we analyzed the influence of vitamin E succinate (5-80 [mu]M), supplemented in the culture medium, on the survival of cultured retinal cells. The release of lactate dehydrogenase (LDH) was decreased in the presence of low concentrations (10-20 [mu]M) of vitamin E succinate, whereas high concentrations (80 [mu]M) induced a significant increase (about 2-fold) in the release of LDH, indicating a red...
| Financiadores do RCAAP | |||||||
|
|
|
|
|
|
||