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A Standardised Static in Vitro Digestion Method Suitable for Food - an Internat...
Minekus, M.; Alminger, M.; Alvito, Paula; Ballance, S.; Bohn, T.; Bourlieu, C.; Carrière, F.; Boutrou, R.; Corredig, M.; Dupont, D.; Dufour, C.Simulated gastro-intestinal digestion is widely employed in many fields of food and nutritional sciences, as conducting human trials are often costly, resource intensive, and ethically disputable. As a consequence, in vitro alternatives that determine endpoints such as the bioaccessibility of nutrients and non-nutrients or the digestibility of macronutrients (e.g. lipids, proteins and carbohydrates) are used fo...
Optimisation, by response surface methodology, of degree of hydrolysis and anti...
Tavares, T. G.; Contreras, M. M.; Amorim, M.; Martín-Álvarez, P. J.; Pintado, M. E.; Recio, I.; Malcata, F. X.The hydrolysis of bovine whey protein concentrate (WPC), a-lactalbumin (a-La) and caseinomacropeptide (CMP), by aqueous extracts of Cynara cardunculus, was optimized using response surface methodology. Degree of hydrolysis (DH), angiotensin-converting enzyme (ACE)-inhibitory activity and antioxidant activity were used as objective functions, and hydrolysis time and enzyme/substrate ratio as manipulated paramete...
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