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Rapid detection of urinary tract infections caused by Proteus spp. using PNA-FISH

Almeida, Carina; Azevedo, N. F.; Bento, Joana C.; Cerca, Nuno; Ramos, H.; Vieira, M. J.; Keevil, C. W.

We developed a fluorescence in situ hybridization (FISH) method for the rapid detection of Proteus spp. in urine, using a novel peptide nucleic acid (PNA) probe. Testing on 137 urine samples from patients with urinary tract infections has shown specificity and sensitivity values of 98 % (95 % CI, 93.2–99.7) and 100 % (95 % CI, 80,8–100), respectively, when compared with CHROMagar Orientation medium. Results ind...


Genotypic and phenotypic differences between nosocomial and commensal Staphyloc...

Freitas, Ana I.; Oliveira, F.; Ramos, H.; Vasconcelos, C.; Vilanova, Manuel; Cerca, Nuno

Staphylococcus epidermidis is a common causative agent of relapsing and persistent hospital-acquired infections, not only because this bacterium belongs to the patients’ microbiota(causing autoinfection) but also because it is easily transmitted by healthcare workers (causing cross-infections). The goal of the present study was to characterize, for the first time, the biofilm-forming ability and virulence assoc...


Thermal analysis of wood-steel hybrid construction

Fonseca, E.M.M.; Ramos, H.M.E.; Silva, H.J.G.; Ferreira, D.R.S.M.

The main objective of this work is to provide the thermal analysis in wood-steel hybrid elements for building constructions under fire conditions. A transient thermal analysis with nonlinear material behaviour will be solved with ANSYS program. The use of wood-steel hybrid models has major advantages as increased fire resistance, and improved high strength. Wood is a lightweight material, easy to assemble, grea...

Data: 2013   |   Origem: Biblioteca Digital do IPB

Thermal analysis of wood-steel hybrid construction

Fonseca, E.M.M.; Ramos, H.M.E.; Silva, H.J.G.; Ferreira, D.R.S.M.

Main goal of this work is to present a numerical model to study the thermal necrosis due a dental drilling process, with and without water irrigation. Also an experimental methodology is used to measure the thermal occurrence in a pig mandible. Motivation, the assessment of bone damage, using the temperature criterion (above 55ºC.

Data: 2013   |   Origem: Biblioteca Digital do IPB

Thermal analysis of wood-steel hybrid construction

Fonseca, E.M.M.; Ramos, H.M.E.; Silva, H.J.G.; Ferreira, D.R.S.M.

The main objective of this work is to provide the thermal analysis in wood-steel hybrid elements for building constructions under fire conditions. A transient thermal analysis with nonlinear material behaviour will be solved with ANSYS program. The use of wood-steel hybrid models has major advantages as increased fire resistance, and improved high strength. Wood is a lightweight material, easy to assemble, grea...

Data: 2013   |   Origem: Biblioteca Digital do IPB

“Uma Boa Morte” – Cirurgia Paliativa na Trissomia 18

Fernandes, CA; Gomes, SM; Neves, CI; Ramos, H; Fernandes, E; Santos, M; Nunes, MA; Magalhães, M; Nascimento, O; Valido, AM

A trissomia 18 caracteriza-se por múltiplas anomalias, incluindo doença cardíaca em 60 a 90% dos casos e elevada mortalidade. O mau prognóstico global, conduz habitualmente a uma politica de “cuidados mínimos” mas, paliar, é também nestas situações, um imperativo ético. Descreve-se o caso de uma recém-nascida sem diagnóstico pré natal, mas com confirmação por cariotipo, com cardiopatia, que condicionou insufici...


Molecular epidemiology of imipenem‐resistant Acinetobacter haemolyticus and Aci...

QUINTEIRA, S.; GROSSO, F.; RAMOS, H.; PEIXE, L.

Antimicrob Agents Chemother. 2007 Sep;51(9):3465-6. Epub 2007 Jul 2. Molecular epidemiology of imipenem-resistant Acinetobacter haemolyticus and Acinetobacter baumannii isolates carrying plasmid-mediated OXA-40 from a Portuguese hospital. Quinteira S, Grosso F, Ramos H, Peixe L. PMID: 17606684 [PubMed - indexed for MEDLINE]


Evolução molecular in vitro: seleção de moléculas inseticidas para insetos-prag...

SILVA, M. C. M. da; BRUNETTA, P. S. F.; FIGUEIRA, E. L. Z.; MAGALHÃES, M. T. Q.; OLIVEIRA, G. R.; RAMOS, H. B.; SARTO, R. P. del; CRUZ, C. M.

O bicudo-do-algodoeiro (Anthonomus grandis) e a lagarta do cartucho (Spodoptera frugiperda) são insetos que causam perdas significativas para a agricultura devido aos danos causados à cultura do algodão. Estratégias biotecnológicas utilizam proteínas envolvidas com a defesa de plantas, incluindo as d-endotoxinas e os inibidores de a-amilases (aAIs), como ferramentas no desenvolvimento de plantas resistentes aos...

Data: 2005   |   Origem: OASIS br

Water enema computed tomography (WE-CT) in the local staging of low colorectal ...

Caseiro-Alves, F; Gonçalo, Manuela; Ilharco, J; Agostinho, A; Castro e Sousa, F; Vilaça-Ramos, H

BACKGROUND: To determine the accuracy of computed tomography performed with a water enema application (WE-CT) in the local staging of low colorectal neoplasms and to compare the results with those of transrectal ultrasonography (TRUS). METHODS: Forty patients with low colorectal tumors were evaluated prospectively by CT with the simultaneous administration of a lukewarm rectal enema (0.5-1.5 L). Thin slices (5 ...


Induced pneumoperitoneum in CT evaluation of peritoneal carcinomatosis

Caseiro-Alves, F; Gonçalo, Manuela; Abraúl, E; Pinto, E; Oliveira, CF; Vilaça-Ramos, H

BACKGROUND: Imaging of peritoneal carcinomatosis is a well-known problem even for technologies as recent as computed tomography (CT). The purpose of this study was to evaluate whether CT performed after induced pneumoperitoneum (CT-PP) could have a higher sensitivity in the detection of peritoneal implants over conventional CT. METHODS: Five patients with known ovarian malignancies underwent standard CT and CT-...


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    Financiadores do RCAAP

Fundação para a Ciência e a Tecnologia Universidade do Minho   Governo Português Ministério da Educação e Ciência Programa Operacional da Sociedade do Conhecimento União Europeia