We developed a fluorescence in situ hybridization (FISH) method for the rapid detection of Proteus spp. in urine, using a novel peptide nucleic acid (PNA) probe. Testing on 137 urine samples from patients with urinary tract infections has shown specificity and sensitivity values of 98 % (95 % CI, 93.2–99.7) and 100 % (95 % CI, 80,8–100), respectively, when compared with CHROMagar Orientation medium. Results ind...
Staphylococcus epidermidis is a common causative agent of relapsing and persistent hospital-acquired infections, not only because this bacterium belongs to the patients’ microbiota(causing autoinfection) but also because it is easily transmitted by healthcare workers (causing cross-infections). The goal of the present study was to characterize, for the first time, the biofilm-forming ability and virulence assoc...
The main objective of this work is to provide the thermal analysis in wood-steel hybrid elements for building constructions under fire conditions. A transient thermal analysis with nonlinear material behaviour will be solved with ANSYS program. The use of wood-steel hybrid models has major advantages as increased fire resistance, and improved high strength. Wood is a lightweight material, easy to assemble, grea...
Main goal of this work is to present a numerical model to study the thermal necrosis due a dental drilling process, with and without water irrigation. Also an experimental methodology is used to measure the thermal occurrence in a pig mandible. Motivation, the assessment of bone damage, using the temperature criterion (above 55ºC.
The main objective of this work is to provide the thermal analysis in wood-steel hybrid elements for building constructions under fire conditions. A transient thermal analysis with nonlinear material behaviour will be solved with ANSYS program. The use of wood-steel hybrid models has major advantages as increased fire resistance, and improved high strength. Wood is a lightweight material, easy to assemble, grea...
A trissomia 18 caracteriza-se por múltiplas anomalias, incluindo doença cardíaca em 60 a 90% dos casos e elevada mortalidade. O mau prognóstico global, conduz habitualmente a uma politica de “cuidados mínimos” mas, paliar, é também nestas situações, um imperativo ético. Descreve-se o caso de uma recém-nascida sem diagnóstico pré natal, mas com confirmação por cariotipo, com cardiopatia, que condicionou insufici...
Antimicrob Agents Chemother. 2007 Sep;51(9):3465-6. Epub 2007 Jul 2. Molecular epidemiology of imipenem-resistant Acinetobacter haemolyticus and Acinetobacter baumannii isolates carrying plasmid-mediated OXA-40 from a Portuguese hospital. Quinteira S, Grosso F, Ramos H, Peixe L. PMID: 17606684 [PubMed - indexed for MEDLINE]
O bicudo-do-algodoeiro (Anthonomus grandis) e a lagarta do cartucho (Spodoptera frugiperda) são insetos que causam perdas significativas para a agricultura devido aos danos causados à cultura do algodão. Estratégias biotecnológicas utilizam proteínas envolvidas com a defesa de plantas, incluindo as d-endotoxinas e os inibidores de a-amilases (aAIs), como ferramentas no desenvolvimento de plantas resistentes aos...
BACKGROUND: To determine the accuracy of computed tomography performed with a water enema application (WE-CT) in the local staging of low colorectal neoplasms and to compare the results with those of transrectal ultrasonography (TRUS). METHODS: Forty patients with low colorectal tumors were evaluated prospectively by CT with the simultaneous administration of a lukewarm rectal enema (0.5-1.5 L). Thin slices (5 ...
BACKGROUND: Imaging of peritoneal carcinomatosis is a well-known problem even for technologies as recent as computed tomography (CT). The purpose of this study was to evaluate whether CT performed after induced pneumoperitoneum (CT-PP) could have a higher sensitivity in the detection of peritoneal implants over conventional CT. METHODS: Five patients with known ovarian malignancies underwent standard CT and CT-...
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