Encontrados 36 documentos, a visualizar página 1 de 4
Inhibition of Mitochondrial Complex III Blocks Neuronal Differentiation and Mai...
Sandro, L. Pereira; Grãos, Mário; Rodrigues, Ana Sofia; Anjo, Sandra I.; Carvalho, Rui A.; Oliveira, Paulo J.; Arenas, Ernest; Ramalho-Santos, JoãoThe mitochondrion is emerging as a key organelle in stem cell biology, acting as a regulator of stem cell pluripotency and differentiation. In this study we sought to understand the effect of mitochondrial complex III inhibition during neuronal differentiation of mouse embryonic stem cells. When exposed to antimycin A, a specific complex III inhibitor, embryonic stem cells failed to differentiate into dopaminer...
p,p′-DDE activates CatSper and compromises human sperm function at environmenta...
Tavares, Renata S.; Mansell, Steven; Barratt, Christopher L. R.; Wilson, Stuart M.; Publicover, Stephen J.; Ramalho-Santos, JoãoThis work was supported by the Portuguese National Science Foundation (FCT) through a grant attributed to the CNC Institution (PEst-C/ SAU/LA0001/2011) and a Wellcome Trust Grant #86470 provided to S.J.P. and C.L.R.B. S.M. was supported by the Infertility Research Trust. R.S.T. is the recipient of a PhD fellowship from FCT (SFRH/ BD/46002/2008).
Energy Metabolism in Human Pluripotent Stem Cells and Their Differentiated Coun...
Varum, Sandra; Rodrigues, Ana S.; Moura, Michelle B.; Momcilovic, Olga; Easley, Charles A.; Ramalho-Santos, João; Van Houten, Bennett; Schatten, GeraldBackground: Human pluripotent stem cells have the ability to generate all cell types present in the adult organism, therefore harboring great potential for the in vitro study of differentiation and for the development of cell-based therapies. Nonetheless their use may prove challenging as incomplete differentiation of these cells might lead to tumoregenicity. Interestingly, many cancer types have been reported ...
In Vitro Surfactant Structure-Toxicity Relationships: Implications for Surfacta...
Inácio, Ângela S.; Mesquita, Katia A.; Baptista, Marta; Ramalho-Santos, João; Vaz, Winchil L. C.; Vieira, Otília V.Background The need for woman-controlled, cheap, safe, effective, easy-to-use and easy-to-store topical applications for prophylaxis against sexually transmitted infections (STIs) makes surfactant-containing formulations an interesting option that requires a more fundamental knowledge concerning surfactant toxicology and structure-activity relationships. Methodology/Principal Findings We report in vitro effect...
Not All Sperm Are Equal: Functional Mitochondria Characterize a Subpopulation o...
Sousa, Ana Paula; Amaral, Alexandra; Baptista, Marta; Tavares, Renata; Caballero Campo, Pedro; Caballero Peregrín, Pedro; Freitas, AlbertinaHuman sperm samples are very heterogeneous and include a low amount of truly functional gametes. Distinct strategies have been developed to characterize and isolate this specific subpopulation. In this study we have used fluorescence microscopy and fluorescence-activated cell sorting to determine if mitochondrial function, as assessed using mitochondrialsensitive probes, could be employed as a criterion to obta...
Mitochondrial bioenergetics of testicular cells from the domestic cat (Felis ca...
Mota, Paula; Amaral, Sandra; Martins, Luís; Pereira, Maria de Lourdes; Oliveira, Paulo J.; Ramalho-Santos, Joãohttp://www.sciencedirect.com/science/article/B6TC0-4VKDMTB-2/2/353c03cb295c4b9b657bd182ae619622
Testicular mitochondrial alterations in untreated streptozotocin-induced diabet...
Amaral, Sandra; Mota, Paula C.; Lacerda, Beatriz; Alves, Marco; Pereira, Maria de Lourdes; Oliveira, Paulo J.; Ramalho-Santos, Joãohttp://www.sciencedirect.com/science/article/B6W8G-4V3HFCC-2/2/73c61572e33e945983a6914b2dfbeb63
Parabens in male infertility--Is there a mitochondrial connection?
Tavares, Renata S.; Martins, Fátima C.; Oliveira, Paulo J.; Ramalho-Santos, João; Peixoto, Francisco P.http://www.sciencedirect.com/science/article/B6TC0-4TRCYC3-1/2/23f0cc8f01dd94bc8f398c89b9855d73
Dual use of Diff-Quik-like stains for the simultaneous evaluation of human sper...
Sousa, Ana Paula M.; Tavares, Renata S.; Velez de la Calle, Juan Felipe; Figueiredo, Helena; Almeida, Vasco; Almeida-Santos, TeresaBACKGROUND: Sperm chromatin status and nuclear DNA damage can be detected using well-established assays. However, most techniques are time-consuming and/or involve elaborate protocols and equipment. We have recently developed a simple and fast method to monitor sperm chromatin status in field conditions using the Diff-Quik assay which is employed in fertility clinics to assess sperm morphology with standard bri...
Seminal traits, suitability for semen preservation and fertility in the native ...
Gamboa, Sandra; Machado-Faria, Manuel; Ramalho-Santos, JoãoThe Puro Sangue Lusitano (PSL) is the major national breed of horse in Portugal, but no studies exist on its seminal characteristics, or on the possibility of conserving semen for future use. The aim of this study was to evaluate semen parameters, fertility and the aptness to semen preservation in Lusitano Stallions. In order to compare characteristics defined by a single or by multiple semen collections per st...
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