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Osteogenic differentiation of two distinct subpopulations of human adipose-deri...
Rada, Tommaso; Santos, T. C.; Marques, A. P.; Correlo, V. M.; Frias, A. M.; Castro, António G.; Neves, N. M.; Gomes, Manuela E.; Reis, R. L.The first stem cells considered for the reconstruction of bone were bone marrow mesenchymal stem cells (BMSCs). Subsequently, cells with similar marker expression panel and differentiation potential were found in new sources of cells, such as adipose tissue. This source of stem cells has a promising future in tissue-engineering applications, considering the abundance of this tissue in the human body, the easy h...
A novel method for the isolation of subpopulations of rat adipose stem cells wi...
Rada, Tommaso; Gomes, Manuela E.; Reis, R. L.Bone marrow has been the elected cell source of studies published so far concerning bone and cartilage tissue-engineering approaches. Recent studies indicate that adipose tissue presents significant advantages over bone marrow as a cell source for tissue engineering. Most of these studies report the use of adipose stem cells (ASCs) isolated by a method based on the enzymatic digestion of the adipose tissue and ...
Distinct stem cells subpopulations isolated from human adipose tissue exhibit d...
Rada, Tommaso; Reis, R. L.; Gomes, Manuela E.Recently adipose tissue has become a research topic also for the searching for an alternative stem cells source to use in cell based therapies such as tissue engineer. In fact Adipose Stem Cells (ASCs) exhibit an important differentiation potential for several cell lineages such as chondrogenic, osteogenic, myogenic, adipogenic and endothelial cells. ASCs populations isolated using standard methodologies (i.e.,...
A novel method for the isolation of subpopulations of rat adipose stem cells wi...
Rada, Tommaso; Gomes, Manuela E.; Reis, R. L.Bone marrow has been the elected cell source of studies published so far concerning bone and cartilage tissue-engineering approaches. Recent studies indicate that adipose tissue presents significant advantages over bone marrow as a cell source for tissue engineering. Most of these studies report the use of adipose stem cells (ASCs) isolated by a method based on the enzymatic digestion of the adipose tissue and ...
Dynamic culture of osteogenic cells in biomimetically coated poly(caprolactone)...
Araújo, J. V.; Reis, C. Cunha; Rada, Tommaso; Silva, Marta Alves da; Gomes, Manuela E.; Yang, Y.; Ashammakhi, N.; Reis, R. L.; El-Haj, Alicia J.In our previous work, biomimetic calcium phosphate-coated poly(caprolactone) nanofibre meshes (BCP-NMs) were demonstrated to be more effective for supporting cell attachment and proliferation under static conditions, when compared with poly(caprolactone) nanofibre meshes (PCL-NMs). In many applications, in vitro cultivation of constructs using bioreactors that support efficient nutrition of cells has appeared a...
Injectable gellan gum hydrogels with autologous cells for the treatment of rabb...
Oliveira, João T.; Gardel, Leandro. S.; Rada, Tommaso; Martins, L.; Gomes, Manuela E.; Reis, R. L.In this work, the ability of gellan gum hydrogels coupled with autologous cells to regenerate rabbit full-thickness articular cartilage defects was tested. Five study groups were defined: (a) gellangumwith encapsulated chondrogenic predifferentiated rabbit adipose stem cells (ASCþGF); (b) gellan gum with encapsulated nonchondrogenic predifferentiated rabbit adipose stem cells (ASC); (c) gellan gum with encapsul...
Expression, purification and osteogenic bioactivity of recombinant human BMP-4,...
Bessa, Paulo C.; Cerqueira, M. T.; Rada, Tommaso; Gomes, Manuela E.; Neves, N. M.; Nobre, A.; Reis, R. L.; Casal, MargaridaBone morphogenetic proteins (BMPs) are cytokines from the TGF-b superfamily, with important roles during embryonic development and in the induction of bone and cartilage tissue differentiation in the adult body. In this contribution, we report the expression of recombinant human BMP-4, BMP-9, BMP-10, BMP-11 (or growth differentiation factor- 11, GDF-11) and BMP-14 (GDF-5), using Escherichia coli pET-25b vector....
Novel method for the isolation of adipose stem cells (ASCs)
Rada, Tommaso; Reis, R. L.; Gomes, Manuela E.Adipose stem cells (ASCs) represent a cell population with great potential for tissue engineering applications. Several articles have been published showing the proliferation and differentiation potential, the markers and the wide range of potential applications of these cells. In the majority of these studies the ASCs are isolated using a basic enzymatic procedure, which results in a quite heterogeneous cell p...
Adipose tissue-derived stem cells and their application in bone and cartilage t...
Rada, Tommaso; Reis, R. L.; Gomes, Manuela E.The adipose tissue was considered a reserve of energy until the ’80s, when it was found that this tissue was involved in the metabolism of sex steroids such as estrogens. From then on, the importance attributed to this tissue radically changed as it was then considered an active organ, involved in important functions of the human body. In 2001, for the first time, the existence of stem cells within this tissue ...
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