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Microtubule cytoskeleton behavior in the initial steps of host cell invasion by...

REIS, Y; CORTES, H; VISEUMELO, L; FAZENDEIRO, I; LEITAO, A; SOARES, H; Cortes, H

Microtubule cytoskeleton behavior in the initial steps of host cell invasion by Besnoitia besnoiti Besnoitia besnoiti is a protozoan parasite responsible for bovine besnoitiosis. Indirect immunofluorescence showed that isolated B. besnoiti possesses a set of subpellicular microtubules, radiating from the apical end and extending for more than 2/3 of the cell body. Upon interaction with the host cell, B. besnoi...


Isolation of Besnoitia besnoiti from infected cattle in Portugal

Cortes, H.C.E.; Reis, Y.; Waap, H.; Vidal, R.; Soares, H.; Marques, I.; Pereira da Fonseca, I.; Fazendeiro, I.; Ferreira, M.L.; Caeiro, V.; Shkap, V.

Abstract Besnoitia besnoiti, an obligate intracellular protozoan parasite belonging to the phylum apicomplexa, is the causative agent of bovine besnoitiosis. Besnoitiosis is responsible for significant losses in the cattle industry of Africa and Mediterranean countries due to the high morbidity rate, abortion and infertility in males. The acute stage of disease is associated with the proliferative forms (tachyz...


Immunodiagnosis of Besnoitia besnoiti infection by ELISA and Western blot

Cortes, H.C.E.; Nunes, S.; Reis, Y.; Staubli, D.; Vidal, R.; Sager, H.; Leitão, A.; Gottstein, B.

Abstract Besnoitia besnoiti, an obligate intracellular apicomplexan protozoan parasite, is the causative agent of bovine besnoitiosis. This infection may dramatically affect body condition and lead to irreversible infertility in males, resulting in important economical losses in livestock production. Identification of serologically positive animals is of major relevance to elaborate appropriate measures of cont...


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