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Study of nitrifying bacteria diversity on biofilms using a molecular approach

Nogueira, R.; Purkhold, Ulrike; Melo, L. F.; Wagner, Michael

The purpose of this work was to investigate the microbial community structure of nitrifying biofilms. Biofilm samples were collected from two reactors operated with distinct retention times. The composition and spatial distribution of nitrifying consortia in biofilms was quantified by fluorescence in situ hybridization (FISH) with rRNA-targeted oligonucleotide probes combined with confocal laser scanning micros...


Nitrifying and heterotrophic population dynamics in biofilm reactors : effects ...

Nogueira, R.; Melo, L. F.; Purkhold, Ulrike; Wuertz, Stefan; Wagner, Michael

Two biofilmreactors operated with hydraulic retention times of 0.8 and 5.0 h were used to study the links between population dynamics and reactor operation performance during a shift in process operation from pure nitrification to combined nitrification and organic carbon removal. The ammonium and the organic carbon loads were identical for both reactors. The composition and dynamics of the microbial consortia ...


Microbial community composition and function in wastewater treatment plants

Wagner, Michael; Loy, Alexander; Nogueira, R.; Purkhold, Ulrike; Lee, Natuschka; Daims, Holger

Biological wastewater treatment has been applied for more than a century to ameliorate anthropogenic damage to the environment. But only during the last decade the use of molecular tools allowed to accurately determine the composition, and dynamics of activated sludge and biofilm microbial communities. Novel, in many cases yet not cultured bacteria were identified to be responsible for filamentous bulking and f...


Microbial population dynamics versus nitrification performance in biofilm react...

Nogueira, R.; Purkhold, Ulrike; Wuertz, Stefan; Melo, L. F.; Wagner, Michael

ln order to improve biological reactors operation and design it is important to study the effects of changes in process parameters with regard to the microbial community structure and, vice versa, the effects of community structure and dynamics on the reactors performance. Two biofilm reactors operated with hydraulic retention times of 1 h and 6 h were used to study the links between population dynamics and rea...


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