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Simultaneous heat and mass transfer in packed bed brying of seeds having a muci...
Prado,M. M.; Sartori,D. J. M.The simultaneous heat and mass transfer between fluid phase and seeds having a mucilaginous coating was studied during packed bed drying. To describe the process, a two-phase model approach was employed, in which the effects of bed shrinkage and nonconstant physical properties were considered. The model took into account bed contraction by employing moving coordinates. Equations relating shrinkage and structura...
Identification and antimicrobial susceptibility of bacteria isolated from corne...
Prado,M.R.; Brito,E.H.S.; Girão,M.D.; Sidrim,J.J.C.; Rocha,M.F.G.A total of 22 clinical specimens were obtained from 19 dogs with corneal ulcer (16 unilateral and three bilateral) for isolation and antimicrobial susceptibility evaluation of the isolated bacteria. Bacterial growth was observed in 100% of the samples (n=22). Staphylococcus intermedius was the predominant species (35.5%), followed by Corynebacterium xerosis (19.3%). Gentamicin, ciprofloxacin, chloramphenicol an...
Nuestra Experiencia en el Tratamiento del Pectus Excavatum Severo Mediante Tora...
Bardají, C.; Maldonado, J.; Royo, Y.; Pueyo, C.; Carrasco, R.; Barbero, L.; Prado, M.RESUMEN Nuestra experiencia en el tratamiento del pectus excavatum severo mediante toracoplastia percutánea videoasistida. Introducción: La deformación congénita más frecuente de la caja torácica es el pectus excavatum (PE): 95% de los casos. PE es una malformación de los cartílagos costales que comporta la respiración paradójica o invertida del paciente desde la fase de la lactancia con hundimiento progresivo ...
Nephrotoxicity of Bence-Jones proteins: interference in renal epithelial cell a...
Nicastri,A.L.; Prado,M.J.B.A.; Dominguez,W.V.; Prado,E.B.A.The aim of the present study was to evaluate the acidification of the endosome-lysosome system of renal epithelial cells after endocytosis of two human immunoglobulin lambda light chains (Bence-Jones proteins, BJP) obtained from patients with multiple myeloma. Renal epithelial cell handling of two BJP (neutral and acidic BJP) was evaluated by rhodamine fluorescence. Renal cells (MDCK) were maintained in culture...
Avaliação do potencial recombinogênico do antibiótico danofloxacina em células ...
Leonardo,A.L.; Castro-Prado,M.A.A.Estudou-se o potencial recombinogênico da danofloxacina, novo antimicrobiano pertencente ao grupo das 4-fluoroquinolonas e de uso exclusivo em medicina veterinária, no fungo filamentoso Aspergillus nidulans. A linhagem mestra UT196 e o mutante Z1 foram utilizados para formar o diplóide Z1//UT196. Conídios desse diplóide foram inoculados em placas de Petri contendo meio mínimo suplementado com 2,5, 5,0 e 10,0 mi...
Use of fluorescent probes to follow membrane traffic in nerve terminals
Guatimosim,C.; Romano-Silva,M.A.; Gomez,M.V.; Prado,M.A.M.Optical tracers in conjunction with fluorescence microscopy have become widely used to follow the movement of synaptic vesicles in nerve terminals. The present review discusses the use of these optical methods to understand the regulation of exocytosis and endocytosis of synaptic vesicles. The maintenance of neurotransmission depends on the constant recycling of synaptic vesicles and important insights have bee...
Non-conventional yeasts as hosts for heterologous protein production.
Domínguez, A.; Fermiñán, E.; Sánchez, M.; Gonzalez, Francisco M.; Pérez-Campo, F. M.; García, S.; Herrero, A.B.; San Vicente, A.; Cabello, J.; Prado, M.Yeasts are an attractive group of lower eukaryotic microorganisms, some of which are used in several industrial processes that include brewing, baking and the production of a variety of biochemical compounds. More recently, yeasts have been developed as host organisms for the production of foreign (heterologous) proteins. Saccharomyces cerevisiae has usually been the yeast of choice, but an increasing number of...
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