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Microplate-based high throughput screening procedure for the isolation of lipid...
Pereira, Hugo; Barreira, Luísa Paula Viola Afonso; Mozes, Andre; Florindo, C.; Polo, Cristina; Duarte, Catarina V; Custódio, Luísa Margarida BatistaAbstract We describe a new selection method based on BODIPY (4,4-difluoro-1,3,5,7-tetramethyl-4-bora-3a,4a-diaza-s-indacene) staining, fluorescence activated cell sorting (FACS) and microplate-based isolation of lipid-rich microalgae from an environmental sample. Our results show that direct sorting onto solid medium upon FACS can save about 3 weeks during the scale-up process as compared with the growth of the...
Data: 2011
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Origem: Sapientia - Universidade do Algarve
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