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A novel aryl acylamidase from Nocardia farcinica hydrolyses polyamide
Heumann, Sonja; Eberl, A.; Fischer-Colbrie, Gudrun; Pobeheim, Herbert; Kaufmann, F.; Ribitsch, D.; Paulo, Artur Cavaco; Gübitz, Georg M.An alkali stable polyamidase was isolated from a new strain of Nocardia farcinica. The enzyme consists of four subunits with a total molecular weight of 190 kDa. The polyamidase cleaved amide and ester bonds of water insoluble model substrates like adipic acid bishexylamide and bis(benzoyloxyethyl)terephthalate and hydrolyzed different soluble amides to the corresponding acid. Treatment of polyamide 6 with this...
New model substrates for enzymes hydrolysing polyethyleneterephthalate and poly...
Heumann, Sonja; Eberel, Anita; Pobeheim, Herbert; Liebminger, Stefan; Fischer-Colbrie, Gudrun; Almansa, Eva; Paulo, Artur Cavaco; Gübitz, Georg M.Recently the potential of enzymes for surface hydrophilisation and/or functionalisation of polyethyleneterephthalate (PET) and polyamide (PA) has been discovered. However, there was no correlation between enzyme class/activity (e.g. esterase, lipase, cutinase) and surface hydrolysis of these polymers and consequently no simple assay to estimate this capability. Enzymes active on the model substrates bis (benzoy...
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