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Evaluation of two methods of in vitro production of ovine embryos using fresh o...
Romão, Ricardo; Marques, Carla; Batista, Maria Conceição; Vasques, Maria Irene; Barbas, J Pedro; Horta, António M; Carolino, NunoSuccessful production of high quality blastocysts depends on the use of a culture system that ensures the acquisition of developmental competence by the maturing oocyte followed by an efficient in vitro fertilization. In the present work the effect of FSH and pyruvate in an EGF containing medium for ovine oocyte maturation prior to insemination with fresh(F)or frozen–thawed (FT) semen on embryo developmental co...
Genetic dissection of the AZF regions of the human Y chromosome: thriller or fi...
Navarro-Costa, Paulo; Plancha, Carlos E.; Gonçalves, JoãoThe azoospermia factor (AZF) regions consist of three genetic domains in the long arm of the human Y chromosome referred to as AZFa, AZFb and AZFc. These are of importance for male fertility since they are home to genes required for spermatogenesis. In this paper a comprehensive analysis of AZF structure and gene content will be undertaken. Particular care will be given to the molecular mechanisms underlying th...
Incorrect DNA methylation of the DAZL promoter CpG island associates with defec...
Navarro-Costa, Paulo; Nogueira, Paulo; Carvalho, Marta; Leal, Fernanda; Cordeiro, Inês; Calhaz-Jorge, Carlos; Gonçalves, João; Plancha, Carlos E.Versão impressa: Hum Reprod. 2010 Oct;25(10):2647-54 ; Background: Successful gametogenesis requires the establishment of an appropriate epigenetic state in developing germ cells. Nevertheless, an association between abnormal spermatogenesis and epigenetic disturbances in germline-specific genes remains to be demonstrated. Methods: In this study, the DNA methylation pattern of the promoter CpG island (CGI) of ...
The AZFc region of the Y chromosome: at the crossroads between genetic diversit...
Navarro-Costa, Paulo; Gonçalves, João; Plancha, Carlos E.BACKGROUND: The three azoospermia factor (AZF) regions of the Y chromosome represent genomic niches for spermatogenesis genes. Yet, the most distal region, AZFc, is a major generator of large-scale variation in the human genome. Determining to what extent this variability affects spermatogenesis is a highly contentious topic in human reproduction. METHODS: In this review, an extensive characterization of the m...
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