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Human skin cell fractions fail to self-organize within a gellan gum/Hyaluronic ...

Split-thickness autografts still are the current gold standard to treat skin, upon severe injuries. Nonetheless, autografts are dependent on donor site availability and often associated to poor quality neoskin. The generation of dermal–epidermal substitutes by tissue engineering is seen as a promising strategy to overcome this problematic. However, solutions that can be safely and conveniently transplanted in o...

Adipose stem cell-derived osteoblasts sustain the functionality of endothelial ...

Vascularization is the most pressing issue in tissue engineering (TE) since ensuring that engineered constructs are adequately perfused after in vivo transplantation is essential for the construct’s survival. The combination of endothelial cells with current TE strategies seems the most promising approach but doubts persist as to which type of endothelial cells to use. Umbilical cord blood (UCB) cells have been...

Biomedical exploitation of squid chitosan using particle aggregation derived co...

Publicado em "Journal of Tissue Engineering and Regenerative Medicine", vol. 7, supp. 1 (2013) ; In the last decades, marine organisms have been the focus of considerable attention as potential source of valuable materials. Some important examples are β-chitin isolated from the endoskeleton of squids and hydroxyapatite (HA) produced from fish-bones. β-chitin is a structural polysaccharide more reactive than th...

Dextran sulphate and ficcol fail as macromolecular crowders to enhance extracel...

Publicado em "Journal of Tissue Engineering and Regenerative Medicine", vol. 7, supp. 1 (2013) ; The development of strategies based on the use of scaffolds for Tissue Engineering and Regenerative Medicine(TERM) has been hindered by the inability of researchers to present solutions to overcome problems mainly related with the biocompatibility of materials andin vivoperfusion of the 3D structures. It is in this...

Human mesenchymal stem cells response to multi-doped silicon-strontium calcium ...

The search for apatitic calcium phosphate coatings to improve implants osteointegration is, nowadays, preferentially focused in the obtaining of compositions closer to that of the inorganic phase of bone. Silicon and strontium are both present in trace concentrations in natural bone and have been demonstrated, by separate, to significantly improve osteoblastic response on calcium phosphate bioceramics. This wor...

Cartilage regeneration approach based on squid chitosan scaffolds : in-vitro as...

During the past decades, marine organisms have been the focus of considerable attention as potential source of valuable materials. For instance, chitosan is a biopolymer with high potential in the biomedical field and can be produced from crustacean shells and squid pens [1]. In this sense, we propose the use of chitosan to produce scaffolds for regenerative medicine purposes. An alkaline solution was used to d...

Cell sheet engineering for reproducing the bone marrow hematopoietic stem cell ...

Hematopoietic stem and progenitor cells (HSPC) are multipotent cells responsible for the maintenance and renewal of the hematopoietic lineage in the adult body. The fate of these stem cells is closely regulated by their surrounding microenvironment, or niche. The importance of the microenvironment for HSPC function has been long recognized by researchers that more than 30 years ago attempted to emulate it in 2D...

Development of osteogenic cell sheets for bone tissue engineering applications

The use of scaffolds in combination with osteogenic cells has been the gold standard in bone tissue engineering strategies. These strategies have, however, in many cases failed to produce the desired results due to issues such as the immunogenicity of the biomaterials used and cell necrosis at the bulk of the scaffold related to deficient oxygen and nutrients diffusion. Here, we originally propose the use of ce...

Co-cultures and cell sheet engineering as relevant tools to improve the outcome...

Taking into consideration the complex biology of bone tissue it is quite clear that the understanding of the cellular interactions that regulate the homeostasis and regeneration of this remarkable tissue is essential for a successful Tissue Engineering strategy. The in vitro study of these cellular interactions relies on co-culture systems, a tremendously useful methodology where two or more cell types are cult...

The potential of osteogenic cell sheets co-cultured with endothelial cells for...