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CD4 T-Cell Cytokine Response to Mite Recombinant Tropomyosin in Mite, Snail and...

Martins, Luís; Pires, Euclides; Inácio, Filipe

This work is a preliminary study on immune cell function, evaluating possible tropomyosin-triggering in a snail-mite-shrimp cross-reaction.


Cardosins improve neuronal regeneration after cell disruption: a comparative ex...

Duarte, Ana; Duarte, Emília; Correia, António; Pires, Euclides; Barros, Marlene

Abstract The establishment of primary cell cultures is invaluable for studying cell and molecular biological questions. Although primary cell cultures more closely resemble and function like in the native environment, during the culture establishment the cells undergo several changes including the damage sustained during their removal from original tissue. The resultant cells have to rebalance the expression o...


Characterization and expression analysis of the aspartic protease gene family o...

Pimentel, Catarina; Straeten, Dominique Van Der; Pires, Euclides; Faro, Carlos; Rodrigues-Pousada, Claudina

Cardosin A and cardosin B are two aspartic proteases mainly found in the pistils of cardoon Cynara cardunculus L., whose flowers are traditionally used in several Mediterranean countries in the manufacture of ewe's cheese. We have been characterizing cardosins at the biochemical, structural and molecular levels. In this study, we show that the cardoon aspartic proteases are encoded by a multigene family. The ge...


Molecular analysis of the interaction between cardosin A and phospholipase Dα

Simões, Isaura; Mueller, Eva-Christina; Otto, Albrecht; Bur, Daniel; Cheung, Alice Y.; Faro, Carlos; Pires, Euclides

Cardosin A is an RGD-containing aspartic proteinase from the stigmatic papillae of Cynara cardunculus L. A putative cardosin A-binding protein has previously been isolated from pollen suggesting its potential involvement in pollen2013pistil interaction [Faro C, Ramalho-Santos M, Vieira M, Mendes A, Simões I, Andrade R, Verissimo P, Lin X, Tang J & Pires E (1999) J Biol Chem274, 28724201328729]. Here we report t...


Metabotropic glutamate and dopamine receptors co-regulate AMPA receptor activit...

Gomes, André R.; Cunha, Paulo; Nuriya, Mutsuo; Faro, Carlos J.; Huganir, Richard L.; Pires, Euclides V.; Carvalho, Ana Luísa; Duarte, Carlos B.

Glutamate receptor phosphorylation has been implicated in several forms of modulation of synaptic transmission. It has been reported that protein kinase A (PKA) can phosphorylate the 03B1-amino-3-hydroxy-5-methyl-4-isoxazolepropionate (AMPA) receptor subunit GluR4 on Ser842, both in vitro and in vivo. Here, we studied the regulation of GluR4 phosphorylation and intracellular trafficking by PKA and by metabotrop...


Thermostability of cardosin A from Cynara cardunculus L.

Pina, David G.; Oliveira, Claudia S.; Sarmento, Ana C.; Barros, Marlene; Pires, Euclides; Zhadan, Galina G.; Villar, Enrique; Gavilanes, Francisco

The structural stability of cardosin A, a plant aspartic proteinase (AP) from Cynara cardunculus L., has been investigated by high-sensitivity differential scanning calorimetry, intrinsic fluorescence and circular dichroism spectroscopy, and enzymatic activity assays. Even though the thermal denaturation of cardosin A is partially irreversible, valid thermodynamic data can be obtained within a wide pH region. A...


Effect of gelation temperature on the properties of skim milk gels made from pl...

Esteves, Cristina L. C.; Lucey, John A.; Hyslop, Douglas B.; Pires, Euclides M. V.

Reconstituted skim milk was gelled at 25-40°C with the plant-origin coagulants from Cynara cardunculus L. or Cynara humilis L. or with fermentation-produced chymosin. Gel formation and ageing were monitored by low amplitude oscillatory rheology and confocal scanning laser microscopy. Arrhenius plots for the rate of milk gelation were also determined. Plant coagulants had shorter gelation time (tg) at 25°C, 35°C...


Protein kinase C gamma associates directly with the GluR4 alpha-amino-3-hydroxy...

Correia, Susana Santos; Duarte, Carlos Bandeira; Faro, Carlos José; Pires, Euclides Vieira; Carvalho, Ana Luísa

Ionotropic glutamate receptors mediate the majority of excitatory synaptic transmission in the brain and are thought to be involved in learning and memory formation. The activity of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA)-type glutamate receptors can be regulated by direct phosphorylation of their subunits, which affects the electrophysiological properties of the receptor, and the receptor ...


Rheological properties of milk gels made with coagulants of plant origin and ch...

Esteves, Cristina L. C.; Lucey, John A.; Pires, Euclides M. V.

The rheological properties of milk gels made using coagulants obtained from the plants Cynara cardunculus L. and Cynara humilis L. were compared with those of fermentation-produced chymosin, using dynamic low amplitude oscillation as well as large strain (yield) testing. Gelation experiments were performed at 32°C using skim milk powder that had been reconstituted for 2 or 16 h at 32°C. The storage modulus (G')...


Molecular cloning and characterization of cDNA encoding cardosin B, an aspartic...

Vieira, Margarida; Pissarra, José; Veríssimo, Paula; Castanheira, Pedro; Costa, Yael; Pires, Euclides; Faro, Carlos

Cardosins A and B are related aspartic proteinases from the pistils of Cynara cardunculus L., whose milk-clotting activity has been exploited for the manufacture of cheese. Here we report the cloning of cardosin B cDNA and its organ, tissue and cytological localization. The cDNA-derived amino acid sequence has 73% similarity with that of cardosin A and displays several distinguishing features. Cardosin B mRNA w...


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    Financiadores do RCAAP

Fundação para a Ciência e a Tecnologia Universidade do Minho   Governo Português Ministério da Educação e Ciência Programa Operacional da Sociedade do Conhecimento União Europeia