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Evaluation of the ability of C. albicans to form biofilm in the presence of pha...

Pires, Diana; Silva, Sónia Carina; Almeida, Carina; Henriques, Mariana; Anderson, Erin M.; Lam, Joseph S.; Sillankorva, Sanna; Azeredo, Joana

Pseudomonas aeruginosa and Candida albicans are disparate microbial species, but both are known to be opportunistic pathogens frequently associated with nosocomial infections. The aim of this study was to provide a better understanding of the interactions between these microorganisms in dual-species biofilms. Several bacteriophage-resistant P. aeruginosa phenotypes have been isolated and were used in dual-speci...


Implementação de um sistema Kanban® na reposição de grandes volumes em dose uni...

Pires, Diana; Ferreira, Sónia; Costa, João; Costa, Ana Fátima; Carvalho, António; Carinha, Paulo Horta

Introdução. O sistema de distribuição em dose unitária tem por base a dispensa de medicação na dose prescrita, de forma individualizada. Este sistema de distribuição é vantajoso, pois torna a dispensa de medicamentos individualizada e dirigida ao doente, e diminui o stock existente nas enfermarias. Contudo, assume também desvantagens, sendo crucial que todos os medicamentos e produtos farmacêuticos estejam disp...


The ability of C. albicans to form biofilm in the presence of sensitive and pha...

Pires, Diana; Silva, Sónia Carina; Henriques, Mariana; Sillankorva, Sanna; Azeredo, Joana

P. aeruginosa and C. albicans are human opportunistic pathogens frequently associated with nosocomial infections. Pathogenic interactions between them have already been identified and it has been reported that C. albicans’ morphology and virulence are significantly affected by the presence of P. aeruginosa. In this work, the interaction between these 2 microorganisms in mixed biofilms was studied. The behavior ...


Can bacteriofages be effective in controlling harmful biofilms?

Sillankorva, Sanna; Pires, Diana; Melo, Luís D. R.; Cerca, Nuno; Azeredo, Joana

(Bacterio)phages are viruses that specifically infect bacteria, causing cell lysis and therefore can be considered a valuable strategy for bacterial control. Recent studies have demonstrated the potential of using phages to control bacterial biofilms. Phages are able to penetrate the extracellular matrnc and can cause up to 90% of biofilm mass reduction even in old biofilms. However phage action can be impaired...


Use of newly isolated phages for the control of Pseudomonas aeruginosa PAO1 and...

Pires, Diana; Sillankorva, Sanna; Faustino, A.; Azeredo, Joana

Pseudomonas aeruginosa is a relevant opportunistic pathogen involved in nosocomial infections. that frequently shows low antibiotic susceptibility. One of its virulence factors is associated with the ability to adhere to surfaces and form virulent biofilms. This work describes the isolation and characterization of lytic phages capable of infecting antibiotic-resistant P. aeruginosa strains. In addition, charact...


Pseudomonas-Candida interaction in dual-species biofilms

Pires, Diana; Silva, Sónia Carina; Henriques, Mariana; Sillankorva, Sanna; Azeredo, Joana


The influence of P. fluorescens cell morphology on the lytic performance and pr...

Sillankorva, Sanna; Pires, Diana; Oliveira, Hugo; Neubauer, P.; Azeredo, Joana

This study aims at assessing the influence of Pseudomonas fluorescence cell morphology on the effectiveness and production of the lytic bacteriophage /IBBPF7A. P. fluorescens were cultured as rods or as elongated cells by varying the temperature and rotary agitation conditions. Cells presented rod shape when grown at temperatures up to 25C and also at 30C under static conditions, and elongated morphology only a...


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Fundação para a Ciência e a Tecnologia Universidade do Minho   Governo Português Ministério da Educação e Ciência Programa Operacional da Sociedade do Conhecimento União Europeia