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A microfluidic device for partial cell separation and deformability assessment
Pinho, D.; Yaginuma, Tomoko; Lima, R.Blood flow in microcirculation shows several interesting phenomena that can be used to develop microfluidic devices for blood separation and analysis in continuous flow. In this study we present a novel continuous microfluidic device for partial extraction of red blood cells (RBCs) and subsequent measurement of RBC deformability. For this purpose, we use polydimethylsiloxane (PDMS) microchannels having differen...
Automatic tracking of labeled red blood cells in microchannels
Pinho, D.; Lima, R.; Pereira, Ana I.; Gayubo, F.The current study proposes an automatic method for the segmentation and tracking of red blood cells flowing through a 100- m glass capillary. The original images were obtained by means of a confocal system and then processed in MATLAB using the Image Processing Toolbox. The measurements obtained with the proposed automatic method were compared with the results determined by a manual tracking method. The compari...
An automatic method to track red blood cells in microchannels
Pinho, D.; Gayubo, F.; Pereira, Ana I.; Lima, R.Image analysis is extremely important to obtain crucial information about the blood phenomena in micro-circulation. The current study proposes an automatic method for segmentation and tracking red blood cells (RBCs) flowing through a 100 μm Glass capillary. The original images were obtained by means of a confocal system and then processed in Matlab using the Image Processing Toolbox. The automatic measurements ...
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