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Can anti-listerial L AB strains, used as starter cultures, affect the sensory p...

Morphological and physiological changes in Saccharomyces cerevisiae by oxidativ...

Increase in air or oxygen pressure in microbial cell cultures can cause oxidative stress and consequently affect cell physiology and morphology. The behaviour of Saccharomyces cerevisiae grown under hyperbaric atmospheres of air and pure oxygen was studied. A limit of 1.0 MPa for the air pressure increase (i.e. 0.21 MPa of oxygen partial pressure) in a fed-batch culture of S. cerevisiae was established. Values ...

Effect of hyperbaric stress on yeast morphology : study by automated image anal...

The effects of hyperbaric stress on the morphology of Saccharomyces cerevisiae were studied in batch cultures under pressures between 0.1 MPa and 0.6 MPa and different gas compositions (air, oxygen, nitrogen or carbon dioxide), covering aerobic and anaerobic conditions. A method using automatic image analysis for classification of S. cerevisiae cells based on their morphology was developed and applied to experi...

Yeast response to hyperbaric stress

Estudo do efeito da pressão na fisiologia de leveduras

Tese de doutoramento em Engenharia Biológica ; A utilização industrial de leveduras requer que as células tenham um metabolismo eficiente, com produtividades elevadas, de modo a que a produção de biomassa ou qualquer outro produto seja o mais económica possível. Durante o decorrer de um processo biotecnológico ocorrem variações das condições operacionais, nomeadamente gradientes de pressão de gases, que provoc...

Fed-batch cultivation of saccharomyces cerevisiae in a hyperbaric bioreactor

Fed-batch is the dominating mode of operation in high-cell-density cultures of Saccharomyces cerevisae in processes such as the production of baker’s yeast and recombinant proteins, where the high oxygen demand of these cultures makes its supply an important and difficult task. The aim of this work was to study the use of hyperbaric air for oxygen mass transfer improvement on S. cerevisiae fed-batch cultivation...

Growth and β-galactosidase activity in cultures of Kluyveromyces marxianus unde...

Aims: To investigate the effect of total air pressure raise on cell growth and intracellular β-galactosidase activity in batch cultures of Kluyveromyces marxianus CBS 7894. Methods and Results: A pressurized bioreactor was used for K. marxianus batch cultivation under increased air pressure from 1.2 to 6 bar. Under these conditions no inhibition of cell growth was observed. Moreover, the improvement of the oxyg...

Growth and beta-galactosidase activity in cultures of Kluyveromyces marxianus u...

Comparison of yeast response to increased pressure at aerobic and anaerobic con...

Oxidative stress response of Kluyveromyces marxianus to hydrogen peroxide, para...

The aim of this work was to study the oxidative stress response of Kluyveromyces marxianus to hydrogen peroxide (50 mM), paraquat (1 mM), an increase in air pressure (120 kPa, 600 kPa) and pure oxygen pressure (120–600 kPa) in a pressurized bioreactor. The effect of these oxidants on metabolism and on the induction of antioxidant enzymes was investigated. The exposure for 1 h of K. marxianus at exponential grow...