Encontrados 6 documentos, a visualizar página 1 de 1
Natural Language Generation in the context of Multimodal Interaction in Portugu...
José Casimiro Pereira; António J. S. Teixeira; Joaquim Sousa PintoIn many situations, systems and people have to interact and share information. With demographic ageing a group of users that deserves special attention are the olderadults. Multimodal interaction aims to improve the interaction between human users and electronic devices. A key technology for the output part of a Multimodal system isconversion of the concepts handled by systems into readable text or audible spee...
Improved kymography tools and its applications to mitosis
Pereira, António J.; Maiato, HelderAlthough applicability of kymographs is limited to nearly one-dimensional (1D) processes, they have been instrumental in the analysis and interpretation of a wide range of dynamic biological processes. We focus here on some applications of kymography in the study of one among the range of ‘nearly-1D’ processes – mitosis. Using this biological context, we suggest generalized procedures in kymograph assembly that...
Synchronizing chromosome segregation by flux-dependent force equalization at ki...
Matos, Irina; Pereira, António J.; Lince-Faria, Mariana; Cameron, Lisa A.; Salmon, Edward D.; Maiato, HelderThe synchronous movement of chromosomes during anaphase ensures their correct inheritance in every cell division. This reflects the uniformity of spindle forces acting on chromosomes and their simultaneous entry into anaphase. Although anaphase onset is controlled by the spindle assembly checkpoint, it remains unknown how spindle forces are uniformly distributed among different chromosomes. In this paper, we sh...
Dynein and Mast/Orbit/CLASP have antagonistic roles in regulating kinetochore-m...
Reis, Rita; Feijão, Tália; Gouveia, Susana; Pereira, António J.; Matos, Irina; Sampaio, Paula; Maiato, Helder; Sunkel, Claudio E.Establishment and maintenance of the mitotic spindle requires the balanced activity of microtubule-associated proteins and motors. In this study we have addressed how the microtubule plus-end tracking protein Mast/Orbit/CLASP and cytoplasmic dynein regulate this process in Drosophila melanogaster embryos and S2 cells. We show that Mast accumulates at kinetochores early in mitosis, which is followed by a polewar...
Dissecting mitosis with laser microsurgery and RNAi in Drosophila cells
Pereira, António J.; Matos, Irina; Lince-Faria, Mariana; Maiato, HelderProgress from our present understanding of the mechanisms behind mitosis has been compromised by the fact that model systems that were ideal for molecular and genetic studies (such as yeasts, C. elegans or Drosophila) were not suitable for intracellular micromanipulation. Unfortunately, those systems that were appropriate for micromanipulation (like newt lung cells, PtK1 cells or insect spermatocytes) are not a...
Mammalian CLASP1 and CLASP2 cooperate to ensure mitotic fidelity by regulating ...
Pereira, Ana L.; Pereira, António J.; Maia, Ana R.R.; Drabek, Ksenija; Sayas, C. Laura; Hergert, Polla J.; Lince-Faria, Mariana; Matos, IrinaCLASPs are widely conserved microtubule plus-end–tracking proteins with essential roles in the local regulation of microtubule dynamics. In yeast, Drosophila, and Xenopus, a single CLASP orthologue is present, which is required for mitotic spindle assembly by regulating microtubule dynamics at the kinetochore. In mammals, however, only CLASP1 has been directly implicated in cell division, despite the existence ...
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