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Detailed morphobiometric studies of Bursaphelenchus xylophilus and characterisa...
Penas, Ana Catarina; Bravo, Maria Antónia; Valadas, Vera; Mota, ManuelDetailed studies on Bursaphelenchus xylophilus are provided in this contribution. Comparative observations between field and cultured populations of this species demonstrated significant size differences: cultured specimens overall displayed larger size in all morphometric parameters. A principal component analysis (PCA) of the individuals undergoing moulting allowed their separation in four groups namely J2-J3...
Species of Bursaphelenchus Fuchs, 1937 (Nematoda: Parasitaphelenchidae) and oth...
Penas, Ana Catarina; Bravo, Maria Antónia; Naves, Pedro; Bonifácio, Luís; Sousa, Edmundo; Mota, ManuelInsects associated with maritime pine, Pinus pinaster, in Portugal were collected and screened for the presence of Bursaphelenchus species. Nematodes were identified using Internal Transcribed Spacers-Restriction Fragment Length Polymorphism (ITS-RFLP) analysis of dauer juveniles and morphological identification of adults that developed from dauer juveniles on fungal cultures or on cultures in pine wood segment...
Bursaphelenchus antoniae sp. n. (Nematoda: Parasitaphelenchidae) associated wit...
Penas, Ana Catarina; METGE, Kai; MOTA, Manuel; VALADAS, VeraBursaphelenchus antoniae sp. n. is described and illustrated. Dauer juveniles were isolated from the body of the large pine weevil, Hylobius sp., collected from maritime pine (Pinus pinaster) stumps, in Portugal. Bursaphelenchus antoniae sp. n. was reared and maintained in P. pinaster wood segments and on Petri dish cultures of the fungi Botrytis cinerea and Monilinia fructicola. The new species is characterise...
Progress in the use of scanning electron microscopy (SEM) applied to the taxono...
Santos, A.R.; VIEIRA, Paulo; MOTA, Manuel; Penas, Ana CatarinaThe scanning electron microscope (SEM) has been a major tool in detailed morphological observations of plant parasitic nematodes during the last 30 years, efficiently complementing light microscopical (LM) studies. Nematodes are extremely difficult to observe and characterize due to their small size (aprox. 1 mm long) and paucity of morphological characters, so detailed surface observations of several organs an...
Species of Bursaphelenchus Fuchs, 1937 (Nematoda: Parasitaphelenchidae) associa...
Penas, Ana Catarina; Correia, Petra; Bravo, Maria Antónia; Mota, Manuel; Tenreiro, RogérioSummary – Species of Bursaphelenchus associated with maritime pine, Pinus pinaster, from Portugal – within and outside the quarantine restricted demarcated zone of B. xylophilus – are described and characterised both morphologically (LM and SEM) and with the use of molecular biology (ITS-RFLP).A new staining method for spicules is proposed. Species include B. hellenicus, B. hylobianum, B. leoni, B. pinophilus, ...
Discovery of PWN in Portugal and in Europe
Mota, Manuel; Bonifácio, L; Bravo, M.A; Naves, Pedro; Penas, Ana Catarina; Pires, J; Sousa, Edmundo; Vieira, PauloPine wood nematode, Bursaphelenchus xylophilus, was first reported for Portugal (and Europe) in 1999. The importance of this discovery and economic impact are discussed. Details on the ongoing research of the nematode and its vector, Monochamus galloprovincialis, are provided, and are mainly on the morphology and molecular biology of the nematode and the bioecology of the vector and its relationship with B. xyl...
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