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The alternative oxidase family of Vitis vinifera reveals an attractive model to...
Costa, José Hélio; Fernandes de Melo, Dirce; Gouveia, Zélia; Cardoso, Hélia Guerra; Peixe, Augusto; Arnholdt-Schmitt, BirgitGenomic design' refers to the structural organization of gene sequences. Recently, the role of intron sequences for gene regulation is being better understood. Further, introns possess high rates of polymorphism that are considered as the major source for speciation. In molecular breeding, the length of gene-specific introns is recognized as a tool to discriminate genotypes with diverse traits of agronomic inte...
PROCESSO DE MICROPROPAGAÇÃO DA OLIVEIRA POR CULTURA IN VITRO DE GOMOS AXILARES
Peixe, AugustoUTILIZANDO A MICROPROPAGAÇÃO IN VITRO DE GOMOS AXILARES, CONSEGUIU-SE A MULTIPLICAÇÃO A BAIXO CUSTO, DA CULTIVAR DE OLIVEIRA ''GALEGA VULGAR'', RECALCITRANTE AOS PROCESSOS CLÁSSICOS DE MULTIPLICAÇÃO VEGETATIVA DA ESPÉCIE (ENXERTIA E ESTACARIA LENHOSA E SEMI-LENHOSA). O PROCESSO CONSISTE NA CULTURA EM CONDIÇÕES DE ASSEPSIA DE GOMOS AXILARES PROVENIENTES DE RAMOS DO ANO DE PLANTAS ADULTAS, UTILIZANDO COMO SUBSTRA...
Coconut water and BAP successfully replaced zeatin in olive (Olea europaea L.) ...
PEIXE, AugustoThe data presented report on trials conducted during 24 months using the Portuguese olive cultivar ‘Galega vulgar’. The effectiveness of coconut water, BAP, or kinetin, as possible zeatin substitutes in olive micropropagation protocols, was investigated. In all stages of the micropropagation process, the mineral and vitamin formulation of Olive Medium (OM) was used. Regarding culture establishment the best resu...
Physiologic responses and gene diversity indicate olive alternative oxidase as ...
Santos Macedo, Elisete; Cardoso, Hélia G.; Hernández, Alejandro; Peixe, Augusto A.; Polidoros, Alexios; Ferreira, Alexandre; Cordeiro, AntónioOlive (Olea europaea L.) trees are mainly propagated by adventitious rooting of semi-hardwood cuttings.However, efficient commercial propagation of valuable olive tree cultivars or landraces by semi-hardwood cuttings can often be restricted by a low rooting capacity. We hypothesize that root induction is a plant cell reaction linked to oxidative stress and that activity of stress-induced alternative oxidase (AO...
Estudo histológico sobre a formação de raízes adventícias em estacas caulinares...
Peixe, Augusto; Serras, Margarida; Campos, Catarina; Zavattieri, Amely; Dias, AlexandraMesmo quando estimulada pela aplicação exógena de reguladores de crescimento, a capacidade de enraizamento da oliveira, por estacaria semi-lenhosa, encontra-se muito dependente do genótipo utilizado. É assim possível encontrar cultivares como a ‘Cobrançosa’, onde se atingem sem dificuldade taxas de formação de raízes adventícias próximas dos 70%, e cultivares como a “Galega vulgar”, onde só muito esporadicament...
AOX – A potential marker for efficient rooting of olive shoot cuttings.
Arnholdt-Schmitt, Birgit; Santos Macedo, Elisete; Cardoso, Hélia Guerra; Peixe, Augusto; Cordeiro, António M.Alternative oxidase (AOX) is recently suggested to be a potential candidate as functional marker for efficient cell reprogramming under stress (Arnholdt-Schmitt et al., 2006a). The presented work is part of a Marie Curie Chair project, that was established to investigate the potential role of the multigene AOX to assist breeding on efficient rooting of olive shoot cuttings (Arnholdt-Schmitt et al. 2006b). Plant...
Induction of haploid morphogenic calluses from in vitro cultured anthers
Peixe, Augusto; Barroso, João; Potes, Amely; Pais, MariaApricot (Prunus armeniaca) ‘Harcot’ anthers were cultured in vitro for the production of haploid plants. The best androgenic response was achieved with Nitsch and Nitsch (1969) medium, supplemented with 4.52 microM 2,4-D, 4.52 micoM zeatin, 2.85 microM IAA and 40 g l−1 sucrose. Cultures were maintained in the dark for 8 days, at 28 ◦C, followed by transfer to a 16-h photoperiod, with 35 micro mm−2 s−1 light int...
Sustained in vitro root development obtained in Pinus pinea inoculated with ect...
Oliveira, Paulo; Barriga, João; Cavaleiro, Cremilde; Peixe, Augusto; Zavattieri, AmelyStone pine (Pinus pinea L.) is an economically important forest tree in Mediterranean climates and has been the target for selection efforts through micropropagation. Previous attempts on microshoots, derived from mature seed cotyledons, reached incipient rooting after induction with a combination of auxin and hypertonic shock, but their development in vitro was not sustained. At this stage, co-culturing pla...
Gamma-irradiated pollen induces the formation of 2n endosperm and abnormal embr...
Peixe, Augusto; Campos, Maria; Cavaleiro, Cremilde; Barroso, João; Pais, MariaThe effect of gamma radiation on pollen germination capacity and pollen tube growth was evaluated in vitro and in situ conditions. In vitro experiments, revealed that irradiation significantly affects pollen viability, mainly for levels higher than 200 Grays (Gy). Also, for levels higher than 200 Gy, in situ observations showed that no pollen tube reached the ovule. Fruit set results confirmed that for irradiat...
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