Encontrados 11 documentos, a visualizar página 1 de 2
African swine fever virus p37 structural protein is localized in nuclear foci c...
Eulálio, Ana; Nunes-Correia, Isabel; Salas, José; Salas, Maria L.; Simões, Sergio; Pedroso de Lima, Maria C.The replication of African swine fever virus DNA is initiated inside the nucleus of host cells, being followed by a longer cytoplasmic replication stage. In face of previous results demonstrating the nucleo-cytoplasmic shuttling activity of ASFV p37 structural protein when considered isolated from the virus infection, we performed a systematic analysis of the subcellular localization of p37 protein in ASFV-infe...
Transfection of oral cancer cells mediated by transferrin-associated lipoplexes...
Neves, Sílvia S.; Sarmento-Ribeiro, Ana B.; Simões, Sérgio P.; Pedroso de Lima, Maria C.The Herpes Simplex Virus thymidine kinase (HSV-tk) suicide gene/ganciclovir (GCV) approach has been used for the treatment of a variety of cancers. The purpose of the present study was to evaluate the cytotoxic effect of ganciclovir in oral squamous cancer cells, previously transfected with HSV-tk gene delivered by transferrin-associated complexes (Tf-lipoplexes), as well as to investigate the mechanisms involv...
Targeting of sterically stabilised pH-sensitive liposomes to human T-leukaemia ...
Fonseca, Cristina; Moreira, João N.; Ciudad, Carlos J.; Pedroso de Lima, Maria C.; Simões, SérgioThe main aim of this work was to develop novel targeted sterically stabilised pH-sensitive liposomes tailored to promote efficient intracellular delivery of therapeutic molecules into human T-leukaemia cells. Our results indicate that the targeting moiety (thiolated transferrin) was successfully coupled to the distal reactive maleimide terminus of poly(ethylene glycol)-phospholipid conjugates incorporated in th...
Improving lipoplex-mediated gene transfer into C6 glioma cells and primary neurons
Girão da Cruz, M. Teresa; Simões, Sérgio; Pedroso de Lima, Maria C.The development of methodologies for gene transfer into the central nervous system is crucial for gene therapy of neurological disorders. In this study, different cationic liposome formulations were used to transfer DNA into C6 glioma cells and primary hippocampal and cortical neurons by varying the nature of the helper lipid (DOPE, Chol) or a mixture of DOPE and cholesterol (Chol) associated to DOTAP. In addit...
On the formulation of pH-sensitive liposomes with long circulation times
Simões, Sérgio; Moreira, João Nuno; Fonseca, Cristina; Düzgünes, Nejat; Pedroso de Lima, Maria C.Strategies used to enhance liposome-mediated drug delivery in vivo include the enhancement of stability and circulation time in the bloodstream, targeting to specific tissues or cells, and facilitation of intracytoplasmic delivery. pH-sensitive liposomes have been developed to mediate the introduction of highly hydrophilic molecules or macromolecules into the cytoplasm. These liposomes destabilize under acidic ...
On the mechanisms of internalization and intracellular delivery mediated by pH-...
Simões, Sérgio; Slepushkin, Vladimir; Düzgünes, Nejat; Pedroso de Lima, Maria C.We investigated the molecular mechanisms by which pH-sensitive liposomes surpass the cytoplasmic and endosomal membranes to deliver their aqueous contents into the cytoplasm. Various liposome formulations were evaluated for their efficacy to mediate intracellular delivery of encapsulated material, including a novel sterically stabilized pH-sensitive formulation ((DOPE:CHEMS:DSPE-PEG2000 (6:4:0.3)) that was prev...
Human serum albumin enhances DNA transfection by lipoplexes and confers resista...
Simões, Sérgio; Slepushkin, Vladimir; Pires, Pedro; Gaspar, Rogério; Pedroso de Lima, Maria C.; Düzgünes, NejatCationic liposome-DNA complexes ([`]lipoplexes') are used as gene delivery vehicles and may overcome some of the limitations of viral vectors for gene therapy applications. The interaction of highly positively charged lipoplexes with biological macromolecules in blood and tissues is one of the drawbacks of this system. We examined whether coating cationic liposomes with human serum albumin (HSA) could generate ...
Interaction of cationic liposomes and their DNA complexes with monocytic leukem...
Pires, Pedro; Simões, Sérgio; Nir, Shlomo; Gaspar, Rogério; Düzgünes, Nejat; Pedroso de Lima, Maria C.Cationic liposomes complexed with DNA have been used extensively as non-viral vectors for the intracellular delivery of reporter or therapeutic genes in culture and in vivo. We examined the relationship between the characteristics of the lipoplexes, their mode of interaction with monocytic THP-1 cells and their ability to transfect these cells. We determined the size and [zeta] potential of cationic liposomes (...
Cholesterol affects African swine fever virus infection
Bernardes, C.; António, A.; Pedroso de Lima, Maria C.; Valdeira, M. L.African swine fever virus (ASFV) enters cells by receptor mediated endocytosis and requires a fusion event between the viral envelope and the limiting membrane of the endosome at low pH. In order to investigate the role of cholesterol in the early stages of ASFV infection, we have studied the effect of the removal of cell and viral membrane cholesterol by cholesterol oxidase treatment of cells and virions, as w...
The influenza virus hemagglutinin: a model protein in the study of membrane fusion
Ramalho-Santos, João; Pedroso de Lima, Maria C.http://www.sciencedirect.com/science/article/B6T22-3T87GFS-5/1/da38b06d6b0db1048937db1795f353fe
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