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Protein micro- and nano-capsules for biomedical applications
Shimanovich, Ulyana; Bernardes, Gonçalo J. L.; Knowles, T. P. J.; Paulo, Artur CavacoMicro- and nano-scale systems have emerged as important tools for developing clinically useful drug delivery systems. In this tutorial review, we discuss the exploitation of biomacromolecules for this purpose, focusing on proteins, polypeptides, nucleic acids and polysaccharides and mixtures thereof as potential building blocks for novel drug delivery systems. We focus on the mechanisms of formation of micro- a...
Odorant binding proteins : a biotechnological tool for odour control
Silva, Carla Manuela; Matamá, Maria Teresa; Azoia, Nuno G.; Mansilha, Catarina; Casal, Margarida; Paulo, Artur CavacoThe application of an odorant binding protein for odour control and fragrance delayed release from a textile surface was first explored in this work. Pig OBP-1 gene was cloned and expressed in Escherichia coli , and the purified protein was biochemically characterized. The IC50 values(concentrations of competitor that caused a decay of fluorescence to half-maximal intensity) were determined for four distinct fr...
The activity of LE10 peptide on biological membranes using molecular dynamics, ...
Antunes, Egipto; Azoia, Nuno G.; Matamá, Maria Teresa; Gomes, Andreia; Paulo, Artur CavacoCell-penetrating peptides (CPPs) and antimicrobial peptides (AMPs) are generally defined as small cationic peptides with the ability to interact with lipidic membranes, in a process driven by electrostatic and hydrophobic processes. The interaction with CPPs is known to lead to its translocation across the membrane, while with AMPs lead to membrane damage. Here we present one synthetic anionic peptide, LE10 (L...
In vitro and computational studies of transdermal perfusion of nanoformulations...
Martins, M.; Azoia, Nuno G.; Ribeiro, Artur J.; Shimanovich, Ulyana; Silva, Carla; Paulo, Artur CavacoTransdermal perfusion of a large protein is reported for the first time, using a nanoemulsion of bovine serum albumin (66 kDa) of 160 nm prepared by a solid-in-oil (S/O) process. Molecular dynamics simulations confirmed skin permeation by these formulations, with integration of the protein into the lipid bilayers. These results demonstrate the real possibility of delivering large proteins transdermally for a ra...
In vitro induction of melanin synthesis and extrusion by tamoxifen
Matamá, Maria Teresa; Araújo, Rita; Preto, Ana; Paulo, Artur Cavaco; Gomes, A. C.Physical appearance has significant importance psychologically and socially, with skin and hair being of prime relevance. Effective ingredients that modulate melanin synthesis are of growing interest. Tamoxifen, a widely used selective oestrogen receptor modulator, SERM, was described occasionally in medical case reports as causing grey hair repigmentation. This work aimed to study, in vitro, the effect of tamo...
The immobilization of polyethylene imine nano and microspheres on glass using h...
Shimanovich, Ulyana; Paulo, Artur Cavaco; Gedanken, AharonThe present article describes the creation and immobilization of Polyethylene imine (PEI) capsules on a glass surface. The synthesis and deposition were accomplished by short-time (3 min) one-step reaction. The preparation and immobilization of PEI spheres, was carried out using an environmental friendly method, the ultrasonic emulsification. The ultrasonic technique enables to control size and fulfillment of t...
Chitosan–lignosulfonates sono-chemically prepared nanoparticles : characterisat...
Kim, Suyeon; Fernandes, Margarida M.; Matamá, Maria Teresa; Loureiro, A.; Gomes, Andreia; Paulo, Artur CavacoDue to their recognised properties of biocompatibility, biodegradability and sustainability, chitosan nanocarriers have been successfully used as new delivery systems. In this work, nanoparticles combining chitosan and lignosulfonates were developed for the first time for cosmetic and biomedical applications. The ability of lignosulfonates to act as a counter polyion for stabilisation of chitosan particles, gen...
Direct enzymatic esterification of cotton and Avicel with wild-type and enginee...
Matamá, Maria Teresa; Casal, Margarida; Paulo, Artur CavacoIn this work, the surface of cellulose, either Avicel or cotton fabric, was modified using cutinases without any previous treatment to swell or to solubilise the polymer. Aiming further improvement of cutinase ester synthase activity on cellulose, an engineered cutinase was investigated. Wild-type cutinase from Fusarium solani and its fusion with the carbohydrate-binding module N1 from Cellulomonas fimi were ab...
Keratins and lipids in ethnic hair
Cruz, Célia F.; Fernandes, Margarida M.; Gomes, Andreia; Coderch, L.; Martí, M.; Méndez, Sandra; Gales, Luís; Azoia, Nuno G.; Shimanovich, UlyanaHuman hair has an important and undeniable relevance in society due to its important role in visual appearance and social communication. Hair is mainly composed of structural proteins, mainly keratin and keratin associated proteins, and lipids. Herein, we report a comprehensive study of the content and distribution of the lipids among ethnic hair, African, Asian and Caucasian hair. More interestingly, we also r...
NMR and molecular modelling studies on elastase inhibitor-peptides for wound ma...
Barros, Sandra Cerqueira; Louro, Ricardo O.; Micaelo, N. M.; Martins, José A.; Marcos, João Carlos; Paulo, Artur CavacoProteases play an important and critical role in the physiological process of wound repair. However, excessive and unregulated release of proteolytic enzymes (e.g., elastase) mediates abnormal degradation of healthy tissues, which leads to inflammatory disorders such as chronic wounds. Thus, it is of therapeutic interest to develop novel synthetic inhibitor-peptides of elastase, which can restore the balance be...
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