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Recurrence After Operative Management of Intrahepatic Cholangiocarcinoma
Hyder, O; Hatzaras, I; Sotiropoulos, G; Paul, A; Alexandrescu, S; Marques, H; Pulitano, C; Barroso, E; Clary, B; Aldrighetti, L; Ferrone, C; Zhu, AINTRODUCTION: Data on recurrence after operation for intrahepatic cholangiocarcinoma (ICC) are limited. We sought to investigate rates and patterns of recurrence in patients after operative intervention for ICC. METHODS: We identified 301 patients who underwent operation for ICC between 1990 and 2011 from an international, multi-institutional database. Clinicopathologic data, recurrence patterns, and recurrenc...
Enterocolite necrosante: experiência de catorze anos
Paul, A; Ferreira, S; Resende, C; Faria, DBackgound: Necrotizing enterocolitis ((NEC) is one of most frequent gastrointestinal emergencies in the neonatal period. Aims: To evaluate the incidência, epidemiology, risk factors and clinical outcomes of newborns with necrotizing enterocolitis admitted to a Level III Neonatal Intensive Care Unit (NICU) . Methods: Casuistic review of newborns with NEC adtmitted to a level III unit, from January 1995 to 31 Dec...
Characterization of bacPPK34 a bacteriocin produced by Pediococcus pentosaceus ...
Abrams, Daniel; Barbosa, Joana; Albano, Helena; Silva, Joana; Gibbs, Paul A.; Teixeira, PaulaDifferent lactic acid bacteria were isolated during different stages in the production of “Alheiras”, a traditionally fermented sausage produced in the north of Portugal, between 2005 and 2007, in a total of 484 isolates. One of 484 isolates (K34) produced a bacteriocin, designated as bacPPK34, and was identified as a strain of Pediococcus pentosaceus by 16S rRNA sequencing. The highest bacteriocin production w...
Numeric taxonomy approaches for lytic evaluation of Salmonella specific bacteri...
Amorim, Lara R. P.; Tamames, Juan A. C.; Silva, Joana G. L.; Gibbs, Paul A.; Teixeira, Paul C.This study explored the lytic ability of bacteriophages as a future tool for reducing the Salmonella spp. loads in food animals. It investigated (a) the concept of a phage cocktail resulting from an exploratory analysis of the 13 phages which were examined, and (b) the possibility of using them in phage typing techniques for a broad range of serotypes. By using the conventional plaque assay method and cluster a...
Characterization of anti-listeria bacteriocins isolated from shellfish: Potenti...
Pinto, Ana Luísa; Fernandes, Melissa; Pinto, Cristina; Albano, Helena; Castilho, Fernanda; Teixeira, Paula; Gibbs, Paul A.This work had as main objectives to characterize two bacteriocins produced by lactic acid bacteria (LAB) previously isolated from non-fermented seafood, in order to evaluate their potential as new food protective agents. The two bacteriocinogenic isolates were identified by Polymerase Chain Reaction (PCR) using genusand species-specific primers, and confirmed by 16S rDNA sequencing, as Enterococcus faecium and ...
Application of an impedimetric technique for the detection of lytic infection o...
Amorim, Lara R. P.; Silva, Joana G. L.; Gibbs, Paul A.; Teixeira, Paula C.This study was performed to evaluate the adaption of the impedimetric method to detect the lytic infection by Salmonella-specific bacteriophages and to provide a higher selectivity to this rapid method in detecting Salmonella spp. by using specific agents. Three bacteriophages and twelve strains of Salmonella spp. were tested. Each of the twelve strains was used separately to inoculate TSB together with each on...
New record of "Bathymodiolus" mauritanicus Cosel 2002 from the Gulf of Cadiz (N...
Genio, Luciana; Johnson, Shannon B.; Vrijenhoek, Robert C.; Cunha, Marina R.; Tyler, Paul A.; Kiel, Steffen; Little, Crispin T. S.The "Bathymodiolus" childressi group is the most geographically diverse assemblage of deep-sea mussel species. In this paper we consider several possible hypotheses to explain the present biogeographic distribution of the "B." childressi species complex. Mussels were collected for the first time from mud volcanoes in the Gulf of Cadiz (NE Atlantic Ocean) during the training through research (TTR) 16 research ex...
A new method to determine the reproductive condition in female tubeworms tested...
Hilario, Ana; Tyler, Paul A.; Pond, David W.Vestimentiferan tubeworms are significant members of deep-sea chemosynthetically-driven communities, including hydrothermal vents and cold seeps. The reproductive condition in this taxon is rarely studied because of sampling constraints inherent to these environments, and the lack of a simple methodology to quantify gonad development in vestimentiferans. We demonstrate that the amount of gonad found in the firs...
Growth control of listeria innocua 2030c on vacuum-packaged cold-smoked salmon ...
Tomé, Elisabetta; Gibbs, Paul A.; Teixeira, Paula C.Five bacteriocin-producing lactic acid bacteria (LAB): Enterococcus faecium ET05, Lactobacillus curvatus ET06, L. curvatus ET30, L. deldrueckii ET32 and Pediococcus acidilactici ET34, selected by their capacity for growth and producing inhibition in vitro at high salt-on-water content, low temperature and anaerobic atmosphere, conditions simulating cold-smoked fish,were inoculated onto salmon fillets, in co-cul...
In vitro tests of suitability of bacteriocin-producing lactic acid bacteria, as...
Tomé, Elisabetta; Pereira, Vera L.; Lopes, Carla I.; Gibbs, Paul A.; Teixeira, Paula C.The objectives of this study were to ascertain the in vitro potential of nine bacteriocin-producing lactic acid bacteria (LAB) strains, isolated from vacuum-packaged cold-smoked salmon (CSS), for possible use as biopreservative cultures against Listeria monocytogenes. The antilisterial activity of cultures’ supernatants was assessed at 0.5%, 3.0% and 5.0% w/v of salt, at 5ºC, 10ºC and 25ºC both in aerobic and a...
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