Collagen, collagen/hyaluronic acid (HA) and collagen/HA/chitosan (CS) sponges loaded with epigallocatechin gallate (EGCG), catechin (CAT) and gallic acid (GA) were developed and evaluated as active chronic wound dressings. Their physico-mechanical properties, biostability, biocompatibility and ability to inhibit in vitro myeloperoxidase (MPO) and collagenase—major enzymes related with the persistent inflammatio...
This work proposes biodegradable textile-based structures for tissue engineering applications. We describe the use of two polymers, polybutylene succinate (PBS) proposed as a viable multifilamentand silk fibroin (SF), to produce fibre-based finely tuned porous architectures by weft knitting. PBS is here proposed as a viable extruded multifilament fibre to be processed by a textile-based technology. A comparativ...
Publicado em "Journal of Tissue Engineering and Regenerative Medicine", vol. 7, supp. 1 (2013) ; Proteins and glycosaminoglycans (GAGs) are the main constituents of the extracellular matrix (ECM). Until very recently, GAGs were considered as pure structural components while proteins have been associated with crucial cell signalling processes. Nowadays, it is well accepted that these two ECM components act in s...
Publicado em "Journal of Tissue Engeneering and Regenerative Medicine", vol. 7, supp. 1 (2013) ; Glycosaminoglycans (GAGs) are integral part of the closest cellular environment: they can be found on the cells surface and in the extracellular matrix, where they interact with different proteins acting as local regulator of their activity. The use of GAGs in the preparation of protein delivery nanosystems is, the...
Glycosaminoglycans (GAGs) comprise the closest cellular environment: they are building elements of the ECM and can be also found on cells surface. Their biological activity depends on several parameters among which the negative charge is of prime importance[1]. This charge is generally associated with the presence of sulfate groups (-OSO3H). Sulfation is a dynamic modification: it can occur at various positions...
Glycosaminoglycans (GAGs) are an integral part of the extracellular matrix and glycocalix, i.e. the closest cellular environment. They are abundant in –OH groups and their bioactivity is also associated with the presence of negatively charged –SO3H functionalities. Therefore, we have investigated and discussed the influence of these functional units on mesenchymal stem cells (MSCs) behaviour using single compon...
The aim of this work was the preparation and characterization of scaffolds with mechanical and functional properties able to regenerate bone. Porous scaffolds made of chitosan/gelatin (POL) blends containing different amounts of a bioactive glass (CEL2), as inorganic material stimulating biomineralization, were fabricated by freeze-drying. Foams with different compositions (CEL2/POL 0/100; 40/60; 70/30 wt %/wt)...
Multilayered polyelectrolyte coatings comprising thiolated chitosan (TC) and glycosaminoglycans (GAGs), chondroitin sulphate and hyaluronic acid, were built using a layer-by-layer approach. The surface activity of these coatings for binding and inhibition of enzymes related to chronic inflammation, such as collagenase and myeloperoxidase, was assessed. The build-up of five bi-layers of TC/GAGs onto gold surface...
Layer-by-layer technique is widely used to produce polyelectrolyte multilayered films for material surface functionalization. The technique simplicity coupled with the biological potential of biopolymers, e.g. polysaccharides, make such assemblies a suitable choice for many biomedical applications. In this study the formation of the films comprising of thiolated chitosan and glycosaminoglycans (GAGs) by alterna...
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