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Adaptive evolution of a lactose-consuming Saccharomyces cerevisiae recombinant
Guimarães, Pedro M. R.; François, J.; Parrou, J. L.; Teixeira, J. A.; Domingues, LucíliaThe construction of Saccharomyces cerevisiae strains that ferment lactose has biotechnological interest, particularly for cheese whey fermentation. A flocculent lactose-consuming S. cerevisiae recombinant expressing the LAC12 (lactose permease) and LAC4 (β-galactosidase) genes of Kluyveromyces lactis was constructed previously but showed poor efficiency in lactose fermentation. This strain was therefore subject...
Adaptive evolution of a lactose-consuming saccharomyces cerevisiae recombinant
Guimarães, Pedro M. R.; François, J.; Parrou, J. L.; Teixeira, J. A.; Domingues, LucíliaThe construction of Saccharomyces cerevisiae strains with the ability to efficiently ferment lactose has biotechnological interest, particularly for the alcoholic fermentation of cheese whey (a high pollutant by-product of dairy industries). A flocculent lactoseconsuming S. cerevisiae recombinant expressing the LAC12 (lactose permease) and LAC4 (beta-galactosidase) genes of Kluyveromyces lactis was previously c...
Adaptive evolution of a recombinant lactose-consuming Saccharomyces cerevisiae ...
Guimarães, Pedro M. R.; Parrou, J. L.; Le Berre, V.; François, J.; Teixeira, J. A.; Domingues, LucíliaIn previous work, a recombinant S. cerevisiae flocculent strain (NCYC869-A3/T1, or simply T1) with the ability to express both the LAC4 (coding for beta-galactosidase) and LAC12 (lactose permease) genes of Kluyveromyces lactis was constructed (Domingues et al., Appl Microbiol Biotechnol 51:621–626, 1999). The original recombinant obtained (T1) was able to metabolise lactose but slowly. Thus, it was subjected to...
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