Encontrados 3 documentos, a visualizar página 1 de 1
Atomic force microscopy characterization of synthetic pyrimidinic oligodeoxynuc...
Paquim, Ana-Maria Chiorcea; Oretskaya, Tatiana S.; Brett, Ana Maria OliveiraIn order to better understand the adsorption mechanism of nucleic acids at electrode surface, MAC Mode atomic force microscopy (MAC Mode AFM) was used to investigate the adsorption of a 10-base synthetic oligodeoxynucleotide (ODN) with the sequence 5'-CTTTTTCTTT-3' containing only pyrimidinic bases, onto a highly oriented pyrolytic graphite (HOPG) electrode. Free adsorption and adsorption at applied potentials ...
Adsorption of synthetic homo- and hetero-oligodeoxynucleotides onto highly orie...
Paquim, Ana-Maria Chiorcea; Oretskaya, Tatiana S.; Brett, Ana Maria OliveiraDNA adsorption on electrode surfaces is of fundamental interest for the development of DNA-based biosensors. The free adsorption of 10-mer synthetic oligodeoxynucleotides (ODNs) onto highly oriented pyrolytic graphite (HOPG) surfaces was studied using Magnetic AC mode atomic force microscopy (MAC Mode AFM). The mechanism of interaction of nucleic acids with carbon electrode surfaces was elucidated, using 10-mer...
DNA imaged on a HOPG electrode surface by AFM with controlled potential
Brett, Ana Maria Oliveira; Paquim, Ana-Maria ChiorceaSingle-molecule AFM imaging of single-stranded and double-stranded DNA molecules self-assembled from solution onto a HOPG electrode surface is reported. The interaction of DNA with the hydrophobic surface induced DNA aggregation, overlapping, intra- and intermolecular interactions. Controlling the electrode potential and using the phase images as a control method, to confirm the correct topographical characteri...
| Financiadores do RCAAP | |||||||
|
|
|
|
|
|
||