Biodesulfurization (BDS) aims at the removal of recalcitrant sulfur from fossil fuels at mild operating conditions with the aid of microorganisms. These microorganisms can remove sulfur from dibenzothiphene (DBT), a model compound, or other polycyclic aromatic used as sulfur source, making BDS an easy and environmental friendly process. Gordonia alkanivorans strain 1B has been described as a desulfurizing bacte...
Chestnut shell s (CS) is an agronomic waste generated from the peeling process of the chestnut fruit. It is well-known that the extract of CS contains high amounts of tannins, which are polyphenolic antioxidants1, but this agronomic residue also contains about 36% sugars in form of polysaccharides, and no utilization of chestnut shells as potential source of fermentable sugars has been considered so far. As con...
Inulin is a carbohydrate composed of linear chains of ß-2,1-linked D-fructofuranose molecules terminated by a glucose residue through a sucrose-type linkage at the reducing end. Jerusalem artichoke (JA) is one of the most interesting materials among unconventional and renewable raw materials, with levels of inulin reaching 50–80% of dry matter. Inulin or inulin-rich materials can be actively hydrolyzed by micro...
Biodesulfurization allows the removal of recalcitrant sulfur from fossil fuels at mild operating conditions with the aid of microorganisms. However the production of biocatalysts still has elevated costs which hinder its industrial application. So the use of agro-industrial by-products and wastes, as alternative carbon sources could present an opportunity to cheapen the process. In previous works we showed tha...
Fructans are one of the most abundant non-structural polysaccharides found in a wide range of plants. Inulin is a polydisperse fructan polymer composed by linear chains of b-2, 1-linked D-fructofuranose molecules terminated by a glucose residue through a sucrose-type linkage at the reducing end. Inulin or inulin-rich materials can be actively hydrolyzed into fermentable sugars (glucose and fructose) using inuli...
This work reports the development of a rapid flow cytometric method for the viability assessment of Gordonia alkanivorans strain 1B, a bacterium used in the biodesulfurization process. To demonstrate that it is possible to monitor by flow cytometric analysis changes in this bacterium physiological state, positive controls using the 5(6)-carboxyfluorescein diacetate (CFDA) and propidium iodide (PI) staining mixt...
Most of the world’s energy is generated from the burning of fossil fuels such as oil and its derivatives. When burnt, these fuels release into the atmosphere volatile organic compounds, sulfur as sulfur dioxide (SO2) and the fine particulate matter of metal sulfates. These are pollutants which can be responsible for bronchial irritation, asthma attacks, cardio-pulmonary diseases and lung cancer mortality, and t...
The removal of sulfur mediated by microorganisms or biodesulfurization (BDS) is already an extensively studied approach. The first studies were reported in the 50’s and 60’s, but only in the last 20 years have been successful breakthroughs. Through BDS it is possible to remove most of the recalcitrant sulfur compounds to the commonly physico-chemical process at mild operating conditions without molecular hydrog...
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