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Late onset congenital adrenal hyperplasia due to 21-hydroxylase deficiency: rev...

The deficiency of the enzyme 21-hydroxylase (21-HO) is responsible for about 90% to 95% of all cases of congenital adrenal hyperplasia (HCSR). This disorder is one of the most frequent hereditary illnesses of autosomal recessive trait. The illness can be presented in two clinical forms: 1 - classic, subdivided in saltwasting form and simple virilizing form; 2 - nonclassic or late-onset. The severity of the illn...

Connecting glucose signalling to ubiquitin-dependent transporter endocytosis : ...

Screening for interactions with predicted soluble domains of the yeast lactate ...

The S. cerevisiae membrane protein Jen1 is a monocarboxylate-proton symporter which after the addition of glucose to lactic acid-grown cells triggers loss of Jen1p activity and repression of JEN1 gene expression (Paiva et al., 2002; Andrade et al., 2001). This downregulation of the permease is dependent on phosphorylation and ubiquitylation mechanisms, both of which are dependent on proteins interacting with th...

A metabolic switch on a yeast arrestin connects glucose signaling to transporte...

Endocytosis is a critical component of plasma membrane dynamics, by allowing the removal of proteins such as transporters or receptors in response to environmental cues. In yeast, transporter endocytosis requires their ubiquitylation at the plasma membrane by the Nedd4-like E3 ubiquitin ligase, Rsp5. Since the ubiquitylation of a given transporter occurs only in response to specific signals, this raises the que...

Plasmids for in vivo construction of integrative Candida albicans vectors in sa...

A general system has been devised for the in vivo construction of Candida albicans integrative vectors in Saccharomyces cerevisiae. The system is especially useful for the integration of genes in C. albicans that cannot be propagated in Escherichia coli possibly because of their toxic effects. The ligation of S. cerevisiae 2μ sequences to a C. albicans integrative vector permits in vivo maintenance and gap repa...

Discovery and initial characterization of members of the new YaaH family of mic...

The emergence of probiotics and prebiotics has revived the importance of short-chain fatty acids (SCFAs) associated to colonic and systemic health improvement. Although biosynthesis and degradation of SCFAs and other short-chain carboxylic acids, such as lactate, pyruvate or citrate are well understood, the transport of these acids is still a matter of discussion. The presence of SCFAs transporters in cellular ...

Functional characterization of a yarrowia lipolytica gene family coding for car...

Carboxylic acids are widely used in pharmaceutical and food industries and new industrial uses are continually emerging. The first step of carboxylic acids metabolism is their entrance to the cell and understanding in detail this process has significant biotechnological relevance. The first carboxylic acids permease found in Saccharomyces cerevisiae was a lactate/H+ symporter coded by JEN1 gene. In Kluyveromyce...

Farmacologia : exercícios teórico práticos

Texto didático desenvolvido no âmbito da Licenciatura em Optometria e Ciências da Visão

Biologia Molecular, bloco 1 : expressão genética : guião das aulas práticas

Azo reductase activity of intact saccharomyces cerevisiae cells Is dependent on...

Unspecific bacterial reduction of azo dyes is a process widely studied in correlation with the biological treatment of colored wastewaters, but the enzyme system associated with this bacterial capability has never been positively identified. Several ascomycete yeast strains display similar decolorizing behaviors. The yeastmediated process requires an alternative carbon and energy source and is independent of pr...