Encontrados 11 documentos, a visualizar página 1 de 2

Anterior positioning of sex chromosomes on the head of human sperm sorted using...

The human ejaculate contains subpopulations of sperm with distinct properties. Human X- and Y-bearing sperm were separated with fluorescence activated cell sorting. To avoid the use of UV light the quantitative DNA dyes DRAQ5® and Dyecycle™ Vybrant® Violet were used. Sorting efficiency was similar for both dyes, but lower than what is usually obtained with the classical method involving Hoechst 33342 and UV lig...

The rs5743836 polymorphism in TLR9 confers a population-based increased risk of...

We are grateful to Paulo Vieira, Cecília Leão, Manuel T. Silva, Nuno Sousa, Jorge Correia- Pinto, Joana Palha, Margarida Correia-Neves, Margarida Lima and Matthew Berry for all their input throughout these studies and critical reading of the manuscript. We are grateful to the patients who joint this study as well as to all members of the Life and Health Sciences Research Institute and School of Health Sciences,...

Not All Sperm Are Equal: Functional Mitochondria Characterize a Subpopulation o...

Human sperm samples are very heterogeneous and include a low amount of truly functional gametes. Distinct strategies have been developed to characterize and isolate this specific subpopulation. In this study we have used fluorescence microscopy and fluorescence-activated cell sorting to determine if mitochondrial function, as assessed using mitochondrialsensitive probes, could be employed as a criterion to obta...

Serratia osteomyelitis and chronic granulomatous disease.

Chronic Granulomatous Disease (CGD) is a primary immunodeficiency disorder characterized by recurrent purulent infections of the skin, lungs, and reticuloendothelial organs, primarily due to staphylococci, enteric bacteria, fungi, and occasionally mycobacteria. More than two thirds of all cases are X-linked and result from defects in the CYBB gene that encodes the gp91-phox subunit of NADPH oxidase. The authors...

Immune changes potentially associated with upper respiratory symptons in swimm...

Eficiência do uso de nutrientes ao longo de uma transição de mata de galeria e ...

Dissertação (mestrado)—Universidade de Brasília, Faculdade de Tecnologia, Departamento de Engenharia Florestal, 2008. ; Na configuração da paisagem que forma o bioma Cerrado, as matas de galeria acompanham os cursos de água e quando fazem limites com o cerrado, diferenciam-se pela posição mais superficial do lençol freático e pelas mudanças significativas quanto às propriedades edáficas. Transições entre for...

S4 13 - PV cell penetrating peptide and cationic liposomes act synergistically...

Cell penetrating peptides have been successfully used to mediate the intracellular delivery of a wide variety of molecules of pharmacological interest. The main aim of the present work was to evaluate the potential of the S413-PV cell penetrating peptide to mediate the intracellular delivery of plasmid DNA, aiming at its use in gene therapy applications. The S413-PV cell penetrating peptide is a chimeric peptid...

Characterization of human sperm populations using conventional parameters, surf...

To directly compare distinct assays proposed to monitor human sperm quality and possibly preselect sperm populations for assisted reproductive technology (ART).Design ; http://www.sciencedirect.com/science/article/B6T6K-4MD46F2-4/1/881ded71ecdcac2b19dc8841945d9b66

Interaction of S413-PV cell penetrating peptide with model membranes: relevance...

Cell penetrating peptides (CPPs) have been successfully used to mediate the intracellular delivery of a wide variety of molecules of pharmacological interest both in vitro and in vivo, although the mechanisms by which the cellular uptake occurs remain unclear and controversial. Following our previous work demonstrating that the cellular uptake of the S413-PV CPP occurs mainly through an endocytosis-independent ...

On the mechanisms of the internalization of S4(13)-PV cell-penetrating peptide

Cell-penetrating peptides have been shown to translocate across eukaryotic cell membranes through a temperature-insensitive and energy-independent mechanism that does not involve membrane receptors or transporters. Although cell-penetrating peptides have been successfully used to mediate the intracellular delivery of a wide variety of molecules of pharmacological interest both in vitro and in vivo, the mechanis...