Encontrados 5 documentos, a visualizar página 1 de 1
A possible approach for gel-based proteomic studies in recalcitrant woody plants
Sebastiana, Mónica; Figueiredo, Andreia; Monteiro, Filipa; Martins, Joana; Franco, Catarina; Coelho, Ana Varela; Vaz, Fátima; Simões, TâniaWoody plants are particularly difficult to investigate due to high phenolic, resin, and tannin contents and laborious sample preparation. In particular, protein isolation from woody plants for two-dimensional gel electrophoresis (2-DE) is challenging as secondary metabolites negatively interfere with protein extraction and separation. In this study, three protein extraction protocols, using TCA, phenol and etha...
Global characterization of the Quercus suber ectomycorrhizal transcriptome usin...
Sebastiana, Mónica; Neto, T. Lino; Tavares, R. M.; Pais, Maria SaloméComunicação em painel ; In temperate forests, trees live in symbiosis with fungi and depend on them for their survival. Species from the Fagaceae family, including oaks, establish a symbiotic relationship in their roots with basidiomycetous fungi, called ectomycorrhizas (ECM). ECM symbiosis is essential for the life and health of trees in temperate and boreal forests where it plays a major role in nutrient cyc...
Optimization of the culture medium composition using response surface methodolo...
Sampaio, Pedro N.; Calado, C.R.C.; Sousa, Lisete; Bressler, David C.; Pais, Maria Salomé; Fonseca, Luís PinaThe optimization of culture medium compositionwas carried out for improvement the recombinantcyprosin B production, an enzyme with high milk-clottingactivity. Response surface methodology (RSM) wasapplied to evaluate the effect of variables namely glucose,yeast extract (YE) and bactopeptone present in the culturemedium, used for recombinant cyprosin B production bytransformed Saccharomyces cerevisiae BJ1991 str...
Involvement of reactive oxygen species during early stages of ectomycorrhiza es...
Baptista, Paula; Martins, Anabela; Pais, Maria Salomé; Tavares, Rui Manuel; Lino-Neto, TeresaEvidence for the participation of reactive oxygen species (ROS) and antioxidant systems in ectomycorrhizal(ECM) establishment is lacking. In this paper, we evaluated ROS production and the activities of superoxide dismutase (SOD) and catalase (CAT) during the early contact of the ECM fungus Pisolithus tinctorius with the roots of Castanea sativa (chestnut tree). Roots were placed in contact with P. tinctorius m...
Estudos e perspectivas futuras das micorrizas e da indução de micorrização in v...
Martins, Anabela; Baptista, Paula; Rodrigues, Paula; Pais, Maria SaloméApresenta-se uma breve retrospectiva dos estudos de micorrização in vitro e ex vitro de plantas de castanheiro micropropagadas e germinantes e os efeitos fisiológicos e químicos da micorrização. Pretende-se ainda perspectivar os estudos em vias de desenvolvimento no domínio da micorrização, dos mecanismos e sinais de associação raiz-fungo e dos efeitos comparativos com associações patogénicas do castanheiro. Es...
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