‘Trincadeira’ and ‘Aragonez’ are two of the most important Vitis vinifera L. cultivars used for red wine production in Portugal. Aiming to obtain a reproducible system for plant genetic transformation, the techniques of particle bombardment and transformation mediated by Agrobacterium tumefaciens were tested. In both systems putative transformed embryos were selected by continued proliferation on an in vitro me...
Somatic embryogenesis induction from in vitro cultured stamens and carpels is highly dependent on explants’ inoculation at specific developmental stages. To establish good correlations between measurable morphometric parameters of flowers or flower buds and developmental stages of micro- and macrosporogenesis, this procedure is the easiest way to simplify the in vitro culture procedures. These correlations were...
Plants encode catalase (CAT; EC 1.11.1.6) as multigene families, which may reflect the multiple and diverse roles played by this enzyme. Catalases from higher plants can be subdivided into three distinct types, according to their phylogenetic relationship. However, there is not a specific correlation of phylogeny and function within these groups, as catalases from the same type can play different specific roles...
The development of the C3 monocot Zantedeschia aethiopica floral spathe has been used as a natural model for studying the physiological and biochemical features of senescence. This photosynthetic leaf-like organ undergoes senescence displaying the common features of leaf senescence, such as loss of protein, chlorophyll and a decline on photosynthetic ability. Fruiting inhibits the ongoing spathe senescence and ...
Poster apresentado no 14th Federation of European Societies of Plant Biology Congress, em 2004, Cracóvia, Polónia. ; Zantedeschia aethiopica spathe undergoes whitening displaying the common features of foliar senescence. After pollination, the spathe region surrounding the fruits undergo regreening, thus reacquiring photosynthetic ability. In this work, changes in peroxisomal metabolism were studied in what co...
Apresentação efectuada no Oxidative Stress and Cell Signalling IBMC Advanced Course, 27 a 29 Outubro 2004, Porto. ; Plant senescence is a natural regulated and programmed process that leads ultimately to cell death. Numerous studies in different plant species have suggested a role for reactive oxygen species (ROS) in the progress of senescence programs, which is frequently associated with the degradative aspec...
Zantedeschia aethiopica spathe undergoes whitening displaying the common features of foliar senescence. After pollination, the spathe region surrounding the fruits undergo regreening, thus reacquiring photosynthetic ability. In this work, changes in peroxisomal metabolism were studied in what concerns key-enzymes of glyoxylate and glycolate pathways. Putative roles for CAT1 and CAT2 are discussed. Material and ...
As micorrizas são o resultado de um processo de co-evolução entre plantas e fungos. As plantas aquáticas primitivas colonizaram o meio ambiente terrestre mercê de um conjunto de condições bióticas e abióticas, de entre as quais se contam a formação de micorrizas. As associações simbióticas são uma regra e não uma excepção entre os organismos mais complexos. As infecções fúngicas mais frequentes no Reino Plantae...
31P-Nuclear Magnetic Resonance (NMR) was used to assess phosphate distribution in ectomycorrhizal and nonmycorrhizal roots of Castanea sativa Mill. as well as in the mycorrhizal fungus Pisolithus tinctorius in order to gain insight into phosphate trafficking in these systems. The fungus P. tinctorius accumulated high levels of polyphosphates during the rapid phase of growth. Mycorrhizal and nonmycorrhizal root...
Mycorrhizal micropropagated Castanea sativa plants were studied in terms of growth and physiological parameters following in vitro mycorrhization with Pisolithus tinctorius. Mycorrhization enhanced growth of micropropagated chestnut plants, increased their protein content and photosynthetic rates, decreased the respiratory rates and CO2 compensation point. RuBisCO activity was not significantly different in myc...
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