Background: Candida parapsilosis is frequently isolated from hospital environments, like air and surfaces, and causes serious nosocomial infections. Molecular studies provided evidence of great genetic diversity within the C. parapsilosis species complex but, despite their growing importance as pathogens, little is known about their potential to cause disease, particularly their interactions with phagocytes. In...
1. Introduction In most cultures, bread making depends on a fermentation step. The flour leavening ability was, at first, most probably dependent on spontaneous fermentation. It became a controlled process by the maintenance of fresh innocula from one preparation to the next and this kind of environmental constraints eventually generated a particular type of yeast and bacteria biodiversity, adapted to ferment ...
Matrix-assisted laser desorption/ionization time-of-flight intact cell mass spectrometry (MALDI-TOF-ICMS) was used to differentiate pathogenic Candida species, difficult to identify by traditional methods such as growth and biochemical reactions. Results showed that species complexes like C. parapsilosis, C. orthopsilosis, and C. metapsilsosis, and very closely related species like C. glabrata and C. bracarensi...
Among the Candida species causing bloodstream infections, Candida parapsilosis is one of the most frequently isolated. The objective of the present work was the identification of new microsatellite loci able to distinguish among C. parapsilosis isolates. DNA sequences with trinucleotide repeats were selected from the C. parapsilosis genome database. PCR primer sets flanking the microsatellite repeats were desig...
The incidence of Candida albicans infections and the relapse episodes after antifungal treatment have increased in recent decades. Recurrences are mainly due to the persistence of the original infecting strain that may present genetic and genomic rearrangements during interaction with the host, reflecting strain adaptation. In this study, four isolates recovered from a patient during recurrent candidemia episod...
The analysis of six polymorphic microsatellite loci was performed in 361 Saccharomyces cerevisiae isolates obtained from spontaneous fermentations. This population derived from a previous screening (using mtDNA RFLP) of 1620 isolates of grapes collected in three vineyards of the Vinho Verde Region, in northwest Portugal, during the 2001 – 2003 harvest seasons. Among the 93 alleles obtained, 52 new alleles were...
Poster apresentado nas "XXXI Jornadas Portuguesas de Genética", em Oeiras, Portugal, em 2004. ; An extensive study was performed in order to better understand the biogeography and biodiversity of Saccharomyces cerevisiae strains isolated from the enological fermentative microflora of the Vinho Verde Wine Region. The 930 isolates collected during 2001 and 2002 from 6 different sampling points and grape maturati...
Resumo da comunicação apresentado na conferência Candida and Candidiasis realizada em Março de 2004 em Austin, Texas EUA.
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