Encontrados 10 documentos, a visualizar página 1 de 1

Modifying fish gelatin electrospun membranes for biomedical applications: cross...

Development of suitable membranes is a fundamental requisite for tissue and biomedical engineering applications. This work presents fish gelatin random and aligned electrospun membranes cross-linked with glutaraldehyde (GA). It was observed that the fiber average diameter and the morphology is not influenced by the GA exposure time and presents fibers with an average diameter around 250 nm. Moreover, when the g...

Processing and characterization of alpha-elastin electrospun membranes

Elastin isolated from fresh bovine ligaments was dissolved in a mixture of 1,1,1,3,3,3-Hexafluoro-2-propanol and water and electrospun into fiber membranes under different processing conditions. Fiber mats of randomly and aligned fibers were obtained with fixed and rotating ground collectors and fibrils were composed by thin ribbons whose width depends on electrospinning conditions; fibrils with 721 nm up to 2....

Thermal and hydrolytic degradation of electrospun fish gelatin membranes

The thermal and hydrolytic degradation of electrospun gelatin membranes cross-linked with glutaraldehyde in vapor phase has been studied. In vitro degradation of gelatin membranes was evaluated in phosphate buffer saline solution at 37 °C. After 15 days under these conditions, a weight loss of 68% was observed, attributed to solvation and depolymerization of the main polymeric chains. Thermal degradation kineti...

Antimicrobial and antioxidant linen via laccase-assisted grafting

A laccase from Ascomycetemyceliophthora thermophila was used to assist the binding of chitosan and catechin onto a previous enzymatically oxidized linen surface. The process consists of the pre-treatment of the linen with laccase followed by the application of chitosan in a first step and catechin plus laccase in a second step. The results presented here support the conclusion that laccase is able to oxidize ph...

Bacterial cellulose : production and applications

Exploiting the sequence of naturally occurring elastin : construction, producti...

Genetic engineering was used to produce an elastin-like polymer (ELP) with precise amino acid composition, sequence and length, resulting in the absolute control of MW and stereochemistry. A synthetic monomer DNA sequence encoding for (VPAVG)20, was used to build a library of concatemer genes with precise control on sequence and size. The higher molecular weight polymer with 220 repeats of VPAVG was biologi...

Construction and bioproduction of a "green" synthetic protein-based polymer exh...

Response surface optimization of the culture medium for production of elastin-l...

Biologia Molecular, bloco 1 : expressão genética : guião das aulas práticas

Physical and chemical optimization of a synthetic medium for Pichia pastoris gr...

The methylotrophic yeast Pichia pastoris is widely used as a host strain for the production of a variety of heterologous proteins. The basal salt medium (BSM) described by Invitrogen (2000) is one of the most broadly used media for this expression system, although it possesses many problems, namely unbalanced composition, salt precipitation and undesirable ionic strength. This medium also uses NH4OH simultan...