Catalase-peroxidase (CP) production by a Bacillus sp. (Bacillus KF) newly isolated from a textile finishing effluent and by this strain immobilised on light expanded clay was studied. In cultivations of Bacillus KF increased catalase activity (about 30-fold) was measured after the addition of H2O2 and Orange IV, while ascorbic acid, pyrogallol and Paraquat, seemed to be poor inducers. Catalases in the cell extr...
Three thermoalkaliphilic bacteria, which were grown at pH 9.3–10 and 60–65 °C were isolated out of a textile wastewater drain. The unknown micro-organisms were identified as thermoalkaliphilic Bacillus sp. Growth nditions were studied and catalase activities and stabilities compared. Catalases from Bacillus SF showed high stabilities at 60 °C and pH 9 (t1/2=38 h) and thus this strain was chosen for further inv...
A catalase preparation from a newly isolated Bacillus sp. was covalently immobilized on silanized alumina using glutaraldehyde as crosslinking agent. The effect of the coupling time of the enzyme-support reaction was determined in terms of protein recovery and immobilization yield and a certain balance point was found after which the activity recovery decreased. The activity profile of the immobilized catalase ...
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