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Synthetic oligonucleotides: AFM characterisation and electroanalytical studies
Brett, A. M. Oliveira; Paquim, A.-M. Chiorcea; Diculescu, V.; Oretskaya, T. S.One of the most important steps in designing more sensitive and stable DNA based biosensors is the immobilisation procedure of the nucleic acid probes on the transducer surface, while maintaining their conformational flexibility. MAC Mode AFM images in air demonstrated that the oligonucleotide sequences adsorb spontaneously on the electrode surface, showing the existence of pores in the adsorbed layer that reve...
AFM and electroanalytical studies of synthetic oligonucleotide hybridization
Paquim, A. -M. Chiorcea; Diculescu, V. C.; Oretskaya, T. S.; Brett, A. M. OliveiraThe first and most important step in the development and manufacture of a sensitive DNA-biosensor for hybridization detection is the immobilization procedure of the nucleic acid probe on the transducer surface, maintaining its mobility and conformational flexibility. MAC Mode AFM images were used to demonstrate that oligonucleotide (ODN) molecules adsorb spontaneously at the electrode surface. After adsorption,...
Electrochemical sensing of the behaviour of oligonucleotide lipoplexes at charg...
Piedade, J. A. P.; Mano, M.; Lima, M. C. Pedroso de; Oretskaya, T. S.; Oliveira-Brett, A. M.Complexes between short oligodeoxynucleotides (ODN) with a variable dGxdCy base composition and liposomes composed of the cationic lipid DOTAP (ODN lipoplexes) were studied by differential pulse voltammetry at a glassy carbon electrode. Since lipoplexes are spontaneously formed by electrostatic interactions, the objective of the voltammetric study was to investigate their behaviour at the electrode surface/solu...
Voltammetric behaviour of oligonucleotide lipoplexes adsorbed onto glassy carbo...
Piedade, J. A. P.; Mano, M.; Lima, M. C. Pedroso de; Oretskaya, T. S.; Oliveira-Brett, A. M.The voltammetric behaviour of oligonucleotide lipoplexes (ODN-lipoplexes) prepared from short oligodeoxynucleotides (ODN), with different base compositions, and liposomes of the cationic lipid DOTAP, was studied by differential pulse voltammetry with a glassy carbon mini-electrode. It was found that the ODN base composition influences the ODN-lipoplex voltammetric response. Differential pulse voltammograms for ...
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