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Analysis of DNA damage and repair in Saccharomyces cerevisiae using the comet a...

In this work we used the model organism Saccharomyces cerevisiae to characterise the biological activity and the mechanism of action of phytochemicals. We have assessed DNA damage and repair using the comet assay, which displayed a dose-response relationship with different DNA-damaging agents. Subsequently, we used this system to assess the antigenotoxic properties of a leaf extract from Ginkgo biloba (GBE). Ex...

Ferramentas de Engenharia Genética : protocolos das aulas práticas

Genes e genomas : protocolos das aulas práticas

Measuring oxidative DNA damage and DNA repair using the yeast comet assay

Chromosomal DNA damage can be a result of several processes and agents of endogenous or exogenous origin. These cause strand breaks or oxidized bases that lead to strand breaks, which relax the normally supercoiled genomic DNA and increase its electrophoretic mobility. The extent of DNA damage can be assessed by single cell gel electrophoresis, where the chromosomal DNA migration distance correlates with the ex...

Analysis of DNA damage and repair in saccharomyces cerevisiae using the comet a...

In this work we used the model organism Saccharomyces cerevisiae to characterise the biological activity and the mechanism of action of phytochemicals. One of the goals is to use mutant strains affected in basic mechanisms of oxidative stress response and DNA repair in order to uncover the molecular targets of phytochemicals. We have assessed DNA damage and repair using the comet assay, evaluated as “comet tail...

Evaluation and characterization of antioxidant and antigenotoxic properties of ...

Propolis is a substance produced by bees (Apis mellifera L.) after harvest of buds and bark of plants and by subsequent mixing with the salivary enzyme β-glucosidase. Bees use propolis in their combs as protection, to repair damage, to build aseptic locals for the eggs of the queen, and also as a thermal insulator. The composition of propolis varies geographically, with the available flora, the time of collecti...

Evaluation of antigenotoxic effects of phytochemicals : DNA protection from oxi...

In recent years, plant extracts and their phytochemicals have received increasing attention, especially in the field of pharmaceutical sciences and medicine, due to the potential prevention activity of a number of chronic and degenerative diseases including cancer and cardiovascular diseases. Most of the health-promoting effects have been attributed to antioxidant action including the ability to scavenge reacti...

Evaluation and characterization of antioxidant and antigenotoxic properties of ...

Propolis is a substance produced by bees (Apis mellifera L.), after harvesting exsudates of plant buds and barks, and subsequent mixing with the salivary enzyme β-glucosidase. Bees use propolis in their combs as protection, to repair damage, to build aseptic locals for the eggs of the queen, and also as a thermal insulator. The composition of propolis varies geographically, with the available flora, the time of...

Analysis of DNA damage and repair in saccharomyces cerevisiae using the comet a...

Apresentação efectuada no 2nd Annual Workshop of the PhD program BioPlant, em Braga, 2011 ; In this work we used the model organism Saccharomyces cerevisiae to characterise the biological activity and the mechanism of action of phytochemicals. We have assessed DNA damage and repair using the comet assay, evaluated as “comet tail length” and used this system to assess the antigenotoxic properties of a leaf extr...

DNA damage repair ability in cell cycle arrested saccharomyces cerevisiae cells

Cells are exposed permanently to different types of environmental stress and to stress caused by oxidative metabolism in mitochondria that target macromolecules like DNA. Although cells dispose a range of stress response mechanisms, balance between DNA damage-causing stress and DNA repair capacity is crucial. Cells have evolved molecular repair mechanisms in case of DNA damage so that genome stability is maint...