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Sistema e método de cultura de plantas in vitro para análise de metabolitos lib...

Castro, Mário Rui da Costa Basílio e; Ragonezi Gomes Lopes, Carla Aparecida; Oliveira, Paulo Guilherme de; Zavattieri, Maria Amely

O presente invento diz respeito ao desenvolvimento de um sistema e método de cultura in vitro (1,2) constituído por duas fases, uma sólida (3,4,5) e uma líquida (6), a primeira para suporte da planta (7,8) e a segunda para permitir a análise do meio circundante das raízes (9). Este sistema foi desenhado para permitir uma fácil extracção dos compostos metabólicos libertados/difundidos na fase líquida durante o c...


Mycorrhiza-like structures in rooted microshoots of Pinus pinea L.

Castro, Mário Rui da Costa Basílio e; Ragonezi Gomes Lopes, Carla Aparecida; Klimaszewska, Krystyna; Lima, Mónica; Oliveira, Paulo Guilherme de

Pinus pinea L. (stone pine) is one of the major plantation species in Iberian Peninsula, being Portugal the largest edible seed producer in the world. The induction and improvement of in vitro rhizogenesis of microshoots of Pinus pinea was developed in our laboratory using a co-culture system with ECM fungi. In the acclimation phase in mixed substrates, or in rhizotrons, anatomical and morphological studies wer...


Adventitious rooting of conifers: influence of physical and chemical factors

Ragonezi, Carla; Klimaszewska, Krystyna; Castro, Mário Rui da Costa Basílio e; Lima, Mónica; Oliveira, Paulo Guilherme de; Zavattieri, Maria Amely

In conifers, vegetative propagation of superior genotypes is the most direct means for making large genetic gains, because it allows a large proportion of genetic diversity to be captured in a single cycle of selection. There are two aims of vegetative propagation, namely large-scale multiplication of select genotypes and production of large numbers of plants from scarce and costly seed that originates from con...


Effects of carbon source, carbon concentration and culture conditions on in vit...

Zavattieri, Maria Amely; Lima, Mónica; Sobral, Virginia; Oliveira, Paulo Guilherme de; Costa, Alexandra Rosa da

In stone pine (Pinus pinea L.), clonal propagation via adventitious shoot formation from cotyledons has been reported before but rooting of these shoots is poor. The number of rooted shoots had low frequency limiting the used of micropropagation protocols as a choice for mass propagation of superior genotypes. Therefore the main objective of the present work was to increase the number and quality of roots per s...


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Fundação para a Ciência e a Tecnologia Universidade do Minho   Governo Português Ministério da Educação e Ciência Programa Operacional da Sociedade do Conhecimento União Europeia