Encontrados 11 documentos, a visualizar página 1 de 2
Comparison of [3,4-13C2] glucose as a tracer for glucose turnover by nuclear m...
Jin, Eunsook S.; Jones, John G.; Burgess, Shawn C.; Merritt, Matthew E.; Sherry, A. Dean; Malloy, Craig R.A recently introduced tracer, [3,4-13C2] glucose, was compared to the widely used tracer, [6,6-2H2]glucose, for measurement of whole-body glucose turnover. The rate of glucose production (GP) was measured in rats after primed infusions of [3,4-13C2]glucose, [6,6-2H2]glucose, or both tracers simultaneously followed by a constant infusion of tracer(s) over 90 min. Blood glucose was purified and converted into mon...
Glucose production, gluconeogenesis, and hepatic tricarboxylic acid cycle fluxe...
Jin, Eunsook S.; Jones, John G.; Merritt, Matthew; Burgess, Shawn C.; Malloy, Craig R.; Sherry, A. DeanA triple-tracer method was developed to provide absolute fluxes contributing to endogenous glucose production and hepatic tricarboxylic acid (TCA) cycle fluxes in 24-h-fasted rats by 2H and 13C nuclear magnetic resonance (NMR) analysis of a single glucose derivative. A primed, intravenous [3,4-13C2]glucose infusion was used to measure endogenous glucose production; intraperitoneal 2H2O (to enrich total body wat...
Compartmentation of glycolysis and glycogenolysis in the perfused rat heart
Anousis, Nick; Carvalho, Rui A.; Zhao, Piyu; Malloy, Craig R.; Sherry, A. DeanDeveloping methods that can detect compartmentation of metabolic pathways in intact tissues may be important for understanding energy demand and supply. In this study, we investigated compartmentation of glycolysis and glycogenolysis in the isolated perfused rat heart using 13C NMR isotopomer analysis. Rat hearts previously depleted of myocardial glycogen were perfused with 5.5 mm [U-13C]glucose plus 50 mU/mL i...
A (13)C isotopomer kinetic analysis of cardiac metabolism: influence of altered...
Carvalho, Rui A.; Rodrigues, Tiago B.; Zhao, Piyu; Jeffrey, F. Mark H.; Malloy, Craig R.; Sherry, A. DeanRat hearts were perfused with mixtures of [3-(13)C]pyruvate and [3-(13)C]lactate (to alter cytosolic redox) at low (0.5 mM) or high (2.5 mM) Ca(2+) concentrations to alter contractility. Hearts were frozen at various times after exposure to these substrates, were extracted, and were then analyzed by (13)C NMR spectroscopy. The time-dependent multiplets observed in the (13)C NMR resonances of glutamate in all he...
Noninvasive evaluation of liver metabolism by 2H and 13C NMR isotopomer analysi...
Burgess, Shawn C.; Weis, Brian; Jones, John G.; Smith, Erin; Merritt, Matthew E.; Margolis, David; Dean Sherry, A.; Malloy, Craig R.http://www.sciencedirect.com/science/article/B6W9V-47HS4GT-7/1/a30f3e0c5749c49c708ac1a531e9026c
Hepatic gluconeogenesis and Krebs cycle fluxes in a CCl4 model of acute liver f...
Carvalho, Rui A.; Jones, John G.; McGuirk, Chris; Sherry, A. Dean; Malloy, Craig R.Acute liver failure was induced in rats by CCl4 administration and its effects on the hepatic Krebs cycle and gluconeogenic fluxes were evaluated in situ by 13C NMR isotopomer analysis of hepatic glucose following infusion of [U-13C]propionate. In fed animals, CCl4 injury caused a significant increase in relative gluconeogenic flux from 0.80 ± 0.10 to 1.34 ± 0.24 times the flux through citrate synthase (p < 0.0...
13C Isotopomer Analysis of Glutamate by J-Resolved Heteronuclear Single Quantum...
Burgess, Shawn C.; Carvalho, Rui A.; Merritt, Matthew E.; Jones, John G.; Malloy, Craig R.; Sherry, A. Dean13C NMR isotopomer analysis is a powerful method for measuring metabolic fluxes through pathways intersecting in the tricarboxylic acid cycle. However, the inherent insensitivity of 13C NMR spectroscopy makes application of isotopomer analysis to small tissue samples (mouse tissue, human biopsies, or cells grown in tissue culture) problematic. 1H NMR is intrinsically more sensitive than 13C NMR and can potentia...
Quantitation of Gluconeogenesis by 2H Nuclear Magnetic Resonance Analysis of Pl...
Jones, John G.; Carvalho, Rui A.; Sherry, A. Dean; Malloy, Craig R.We present a simple 2H NMR assay of the fractional contribution of gluconeogenesis to hepatic glucose output following ingestion of 2H2O. The assay is based on the measurement of relative deuterium enrichment in hydrogens 2 and 3 of plasma glucose. Plasma glucose was enzymatically converted to gluconate, which displays fully resolved deuterium 2 and 3 resonances in its 2H NMR spectrum at 14.1 T. The signal inte...
Multiple bond 13C-13C spin-spin coupling provides complementary information in ...
Carvalho, Rui A.; Babcock, Evelyn E.; Jeffrey, F. Mark H.; Sherry, A. Dean; Malloy, Craig R.Most 13C nuclear magnetic resonance (NMR) isotopomer analyses relate a metabolic index of interest to populations of 13C isotopomers as reported by one-bond 13C-13C spin-spin couplings. Metabolic conditions that produce highly enriched citric acid cycle intermediates often lead to 13C NMR spectra of metabolites such as glutamate that show extra multiplets due to long-range couplings. It can be demonstrated from...
13C isotopomer analysis of glutamate by heteronuclear multiple quantum coherenc...
Carvalho, Rui A.; Jeffrey, F. Mark H.; Sherry, A. Dean; Malloy, Craig R.13C has become an important tracer isotope for studies of intermediary metabolism. Information about relative flux through pathways is encoded by the distribution of 13C isotopomers in an intermediate pool such as glutamate. This information is commonly decoded either by mass spectrometry or by measuring relative multiplet areas in a 13C NMR spectrum. We demonstrate here that groups of glutamate 13C isotopomers...
| Financiadores do RCAAP | |||||||
|
|
|
|
|
|
||