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Angolan cymbopogon citratus used for therapeutic benefits: nutritional composit...
Soares, Marta O.; Alves, Rita C.; Pires, Pedro C.; Oliveira, M. Beatriz P. P.; Vinha, Ana F.Folk medicine is a relevant and effective part of indigenous healthcare systems which are, in practice, totally dependent on traditional healers. An outstanding coincidence between indigenous medicinal plant uses and scientifically proved pharmacological properties of several phytochemicals has been observed along the years. This work focused on the leaves of a medicinal plant traditionally used for therapeutic...
Analysis of polycyclic aromatic hydrocarbons in fish: optimisation and validati...
Ramalhosa, Maria João; Paíga, Paula; Morais, Simone; Sousa, Ana M. M.; Gonçalves, Maria Pilar; Delerue-Matos, Cristina; Oliveira, M. Beatriz P. P.An accurate and sensitive method for determination of 18 polycyclic aromatic hydrocarbons (PAHs) (16 PAHs considered by USEPA as priority pollutants, dibenzo[a,l]pyrene and benzo[j]fluoranthene) in fish samples was validated. Analysis was performed by microwave-assisted extraction and liquid chromatography with photodiode array and fluorescence detection. Response surface methodology was used to find the optima...
DNA-based biosensor for the electrocatalytic determination of antioxidant capac...
Barroso, M. Fátima; de-los-Santos-Álvarez, N.; Lobo-Castañón, M. J.; Miranda-Ordieres, A. J.; Delerue-Matos, Cristina; Oliveira, M. Beatriz P. P.Reactive oxygen species (ROS) are produced as a consequence of normal aerobic metabolism and are able to induce DNA oxidative damage. At the cellular level, the evaluation of the protective effect of antioxidants can be achieved by examining the integrity of the DNA nucleobases using electrochemical techniques. Herein, the use of an adenine-rich oligonucleotide (dA21) adsorbed on carbon paste electrodes for the...
Towards a reliable technology for antioxidant capacity and oxidative damage eva...
Barroso, M. Fátima; de-los-Santos-Álvarez, N.; Delerue-Matos, Cristina; Oliveira, M. Beatriz P. P.To counteract and prevent the deleterious effect of free radicals the living organisms have developed complex endogenous and exogenous antioxidant systems. Several analytical methodologies have been proposed in order to quantify antioxidants in food, beverages and biological fluids. This paper revises the electroanalytical approaches developed for the assessment of the total or individual antioxidant capacity. ...
Analysis of polycyclic aromatic hydrocarbons in fish: evaluation of a quick, ea...
Ramalhosa, Maria João; Paíga, Paula; Morais, Simone; Delerue-Matos, Cristina; Oliveira, M. Beatriz P. P.QuEChERS method was evaluated for extraction of 16 PAHs from fish samples. For a selective measurement of the compounds, extracts were analysed by LC with fluorescence detection. The overall analytical procedure was validated by systematic recovery experiments at three levels and by using the standard reference material SRM 2977 (mussel tissue). The targeted contaminants, except naphthalene and acenaphthene, we...
Survey of trace elements (Al, As, Cd, Cr, Co, Hg, Mn, Ni, Pb, Se, and Si) in re...
Barroso, M. Fátima; Ramos, Sandra; Oliva-Teles, Maria Teresa; Delerue-Matos, Cristina; Sales, M. Goreti F.; Oliveira, M. Beatriz P. P.Concentrations of eleven trace elements (Al, As, Cd, Cr, Co, Hg, Mn, Ni, Pb, Se, and Si) were measured in 39 (natural and flavoured) water samples. Determinations were performed using graphite furnace electrothermetry for almost all elements (Al, As, Cd, Cr, Co, Mn, Ni, Pb, and Si). For Se determination hydride generation was used, and cold vapour generation for Hg. These techniques were coupled to atomic absor...
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