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Development and Characterization of a Novel Hybrid Tissue Engineering–Based Sca...
Silva, Nuno; Salgado, A. J.; Sousa, R. A.; Oliveira, João T.; Adriano, Pedro; Leite-Almeida, Hugo; Cerqueira, R.; Almeida, Armando; Mastronardi, F.Spinal cord injury (SCI) represents a significant health and social problem, and therefore it is vital to develop novel strategies that can specifically target it. In this context, the objective of the present work was to develop a new range of three-dimensional (3D) tubular structures aimed at inducing the regeneration within SCI sites. Up to six different 3D tubular structures were initially developed by rapi...
Dynamic culturing of cartilage tissue : the significance of hydrostatic pressure
Correia, Cristina; Pereira, Ana L.; Duarte, Ana Rita C.; Frias, A. M.; Pedro, A. J.; Oliveira, João T.; Sousa, R. A.; Reis, R. L.Human articular cartilage functions under a wide range of mechanical loads in synovial joints, where hydrostatic pressure (HP) is the prevalent actuating force. We hypothesized that the formation of engineered cartilage can be augmented by applying such physiologic stimuli to chondrogenic cells or stem cells, cultured in hydrogels, using custom-designed HP bioreactors. To test this hypothesis, we investigated t...
Gellan gum-based hydrogels for intervertebral disc tissue engineering applications
Correia, Joana Silva; Oliveira, Joaquim M.; Caridade, S. G.; Oliveira, João T.; Sousa, R. A.; Mano, J. F.; Reis, R. L.Intervertebral disc (IVD) degeneration is a challenging clinical problem that urgently demands viable nucleus pulposus (NP) implant materials. The best suited biomaterial for NP regeneration has yet to be identified, but it is believed that biodegradable hydrogel-based materials are promising candidates. In this work, we have developed ionic- and photo-crosslinked methacrylated gellan gum (GG–MA) hydrogels to b...
Injectable gellan gum-based hydrogels for intervertebral disc regeneration
Correia, Joana Silva; Oliveira, Joaquim M.; Caridade, S. G.; Oliveira, João T.; Sousa, R. A.; Mano, J. F.; Reis, R. L.Intervertebral disc (IVD) degeneration is a challenging pathology that, due to the inefficiency of the current treatments, urgently demands for the development of new regenerative approaches[1]. The best viable implant material for nucleus pulposus (NP) regeneration has yet to be identified, but it is believed that biodegradable hydrogelbased materials are promising candidates[2]. In this work, we are proposing...
Development of gellan gum-based microparticles/hydrogel matrices for applicatio...
Pereira, D. R.; Correia, Joana Silva; Caridade, S. G.; Oliveira, João T.; Sousa, R. A.; Salgado, A. J.; Oliveira, Joaquim M.; Mano, J. F.; Sousa, NunoLow back pain is one of the most reported medical conditions associated to intervertebral disc (IVD) degeneration. Nucleus pulposus (NP) is often regarded as the structure where intervertebral disc degeneration begins. Gellan gum-based (GG) hydrogels for acellular and cellular tissue engineering strategies have been developed for finding applications as NP substitutes. The innovative strategy is based on the re...
Development and characterization of a novel hybrid tissue engineering-based sca...
Silva, Nuno; Salgado, A. J.; Sousa, R. A.; Oliveira, João T.; Pedro, A. J.; Almeida, Hugo Leite; Cerqueira, R.; Almeida, Armando; Mastronardi, F.Spinal cord injury (SCI) represents a significant health and social problem, and therefore it is vital to develop novel strategies that can specifically target it. In this context, the objective of the present work was to develop a new range of three-dimensional (3D) tubular structures aimed at inducing the regeneration within SCI sites. Up to six different 3D tubular structures were initially developed by rapi...
Genipin-cross-linked collagen/chitosan biomimetic scaffolds for articular carti...
Yan, Leping; Wang, Yingjun; Rei, Li; Wu, Gang; Caridade, S. G.; Fan, Jiabing; Wang, Lingyun; Ji, Peihong; Oliveira, Joaquim M.; Oliveira, João T.This was the first study to use genipin to cross-link collagen and chitosan. ; In this study, genipin-cross-linked collagen/chitosan biodegradable porous scaffolds were prepared for articular cartilage regeneration. The influence of chitosan amount and genipin concentration on the scaffolds physicochemical properties was evaluated. The morphologies of the scaffolds were characterized by scanning electron micro...
Modified Gellan Gum hydrogels with tunable physical and mechanical properties
Coutinho, Daniela F.; Sant, Shilpa; Shin, Hyeongho; Oliveira, João T.; Gomes, Manuela E.; Neves, N. M.; Khademhosseini, Ali; Reis, R. L.Gellan Gum (GG) has been recently proposed for tissue engineering applications. GG hydrogels are produced by physical crosslinking methods induced by temperature variation or by the presence of divalent cations. However, physical crosslinking methods may yield hydrogels that become weaker in physiological conditions due to the exchange of divalent cations by monovalent ones. Hence, this work presents a new clas...
The effect of insulin-loaded chitosan particle-aggregated scaffolds in chondrog...
Malafaya, P. B.; Oliveira, João T.; Reis, R. L.Osteochondral defect repair requires a tissue engineering approach that aims at mimicking the physiological properties and structure of two different tissues (cartilage and bone) using a scaffold–cell construct. One ideal approach would be to engineer in vitro a hybrid material using a single-cell source. For that purpose, the scaffold should be able to provide the adequate biochemical cues to promote the selec...
Injectable gellan gum hydrogels with autologous cells for the treatment of rabb...
Oliveira, João T.; Gardel, Leandro. S.; Rada, Tommaso; Martins, L.; Gomes, Manuela E.; Reis, R. L.In this work, the ability of gellan gum hydrogels coupled with autologous cells to regenerate rabbit full-thickness articular cartilage defects was tested. Five study groups were defined: (a) gellangumwith encapsulated chondrogenic predifferentiated rabbit adipose stem cells (ASCþGF); (b) gellan gum with encapsulated nonchondrogenic predifferentiated rabbit adipose stem cells (ASC); (c) gellan gum with encapsul...
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