Proceedings of the International Conference “Environmentally friendly and safe technologies for quality of fruit and vegetables”, held in Universidade do Algarve, Faro, Portugal, on January 14-16, 2009. This Conference was a join activity with COST Action 924. ; Quality of fruit and vegetables is defined at harvest, and postharvest technologies only ensure that the rate at which products’ quality decline is mi...
Analysis of the expression of S alleles in ‘Rocha’ pear. In this work, the expression of incompatibility genes (S-alleles) in ‘Rocha’ pear was investigated. The comparison of these alleles with the alleles of other cultivars may constitute a tool to help the technicians and farmers to choose pollinators when planning a new ‘Rocha’ orchard. We have used molecular techniques that include pistil RNA extraction fro...
In order to select pear cultivar which are genetically compatible with ‘Rocha’ (Sa, Sj), the identification of S alleles sequences of commercial cultivars with bloom periods overlapping (partially or totally) the bloom period of ‘Rocha’ was performed. Partial sequences of the S alleles from the different cultivars were amplified with specific primers, cloned and sequenced. ‘Pêra De Água’ (S22, Sb) ‘Général Lecl...
To clarify the role of ammonium transporters in N nutrition of ‘Rocha’ pear trees grafted on ‘BA29’ quince, genes encoding ammonium transporters (AMT1) expressed in the roots were identified under no N supply, and their diversity and regulation upon N application after fruit set was investigated. In this study, thin roots of ‘Rocha’pear/’BA29’ quince were sampled in a plot without N fertilization and four disti...
In order to study the evolution of the quality characteristics of apples cv. Royal Gala under refrigerated air storage, 625 ppb of 1-methylcyclopropene (1-MCP) were applied to the fruits for 24 h at 0 ºC. Fruit samples were analysed after storage and after 7 days at 20 ºC (shelf-life) for titratable acidity, pulp firmness, total soluble solids content and skin colour along a 180 days storage period. The storage...
The effect of 1-methylcyclopropene (1-MCP) treatment on the ripening of ‘Rocha’ pears harvested with different stages of maturity, during a cold storage period of seven months was evaluated. Twenty four hours after harvest, the fruits were treated with 300 ppb of 1-MCP for 24 h at 0 ºC. The fruits were stored under normal atmosphere at 0 ºC and 90-95 % RH. Fruit samples were removed from storage periodically an...
In order to identify genes that are specifically regulated during the expression of the resistance mechanism of the apple cv. Florina towards the apple scab fungus, the genetic expression pattern in plants inoculated with a conidial suspension of Venturia inaequalis was compared with the expression pattern in plants inoculated with water, by differential display RT-PCR. Amplified products derived from the combi...
Fruit softening is thought to result from extensive cellwall modifications that occur during ripening. These modifications are the result, at least in part, of the activity of members of cell wall-modifying enzymes from the same families involved in the cell wall loosening which promote tissue extension and growth. In this work, the activities of a set of pectolytic and non-pectolytic cell wall-modifying enzyme...
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