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Gastroenterite aguda em crianças internadas na área de Lisboa

Escobar, C; Silva, T; Costa, B; Oliveira, M; Correia, P; Ferreira, G; Costa, I; Júlio, C; Rodrigues, J; Machado, J; Marques, A; Simões, MJ; Oleastro, M

Introdução: A Gastroenterite Aguda (GEA) é uma patologia com importante morbilidade sendo a segunda causa de internamento na idade pediátrica. Objetivo: Caracterizar a GEA, em crianças internadas em dois hospitais da área de Lisboa com diferentes características demográficas. Métodos: Estudo prospetivo de maio 2011 a junho 2012. Pesquisados potenciais agentes etiológicos por técnicas convencionais e de biologia...


Routine screening of harmful microorganisms in beach sands: implications to pub...

Sabino, Raquel; Rodrigues, R.; Costa, I.; Carneiro, C.; Cunha, M.; Duarte, A.; Faria, N.; Ferreira, F. C.; Gargaté, M. J.; Júlio, C.; Martins, M. L.

Beaches worldwide provide recreational opportunities to hundreds of millions of people and serve as important components of coastal economies. Beach water is often monitored for microbiological quality to detect the presence of indicators of human sewage contamination so as to prevent public health outbreaks associated with water contact. However, growing evidence suggests that beach sand can harbor microbes ha...


Validation of a PNA-FISH method for the detection of Helicobacter pylori

Cerqueira, L.; Fernandes, RM.; Ferreira, RM.; Oleastro, M.; Carneiro, F.; Brandão, C.; Pimentel-Nunes, P.; Dinis-Ribeiro, M.; Figueiredo, C.

Here, we evaluated a previously established peptide nucleic acid-fluorescence in situ hybridization (PNA-FISH) method as a new diagnostic test for Helicobacter pylori clarithromycin resistance detection in paraffin-embedded gastric biopsy specimens. Both a retrospective study and a prospective cohort study were conducted to evaluate the specificity and sensitivity of a PNA-FISH method to determine H. pylori cla...


The Bactericidal Activity of Carbon Monoxide-Releasing Molecules against Helico...

Tavares, A.F.; Parente, M.R.; Justino, M.; Oleastro, M.; Nobre, L.S.; Saraiva, L.M.

Helicobacter pylori is a pathogen that establishes long life infections responsible for chronic gastric ulcer diseases and a proved risk factor for gastric carcinoma. The therapeutic properties of carbon-monoxide releasing molecules (CORMs) led us to investigate their effect on H. pylori. We show that H. pylori 26695 is susceptible to two widely used CORMs, namely CORM-2 and CORM-3. Also, several H. pylori clin...



Prevalence and risk factors for giardia duodenalis infection among children: a ...

Júlio, C.; Vilares, A.; Oleastro, M.; Ferreira, I.; Gomes, S.; Monteiro, L.; Nunes, B.; Tenreiro, R.; Ângelo, H.

Background Giardia duodenalis is a widespread parasite of mammalian species, including humans. The prevalence of this parasite in children residing in Portugal is currently unknown. This study intended to estimate G. duodenalis infection prevalence and identify possible associated risk factors in a healthy paediatric population living in the District of the Portuguese capital, Lisbon. Methods Between Februar...


Primary antibiotic resistance of helicobacter pylori strains isolated from Port...

Oleastro, M; Cabral, J; Ramalho, P; Lemos, P; Paixão, E; Benoliel, J; Santos, A; Lopes, A

OBJECTIVES: The aim of this study was to prospectively assess the pattern of evolution of primary resistance to antibiotics in Helicobacter pylori strains isolated from Portuguese children over a 10 year period (2000-09). METHODS: A total of 1115 H. pylori strains were tested for antibiotic susceptibility to clarithromycin, metronidazole, amoxicillin, ciprofloxacin and tetracycline. RESULTS: H. pylori strains...


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    Financiadores do RCAAP

Fundação para a Ciência e a Tecnologia Universidade do Minho   Governo Português Ministério da Educação e Ciência Programa Operacional da Sociedade do Conhecimento União Europeia