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Prediction of intracellular storage polymers using quantitative image analysis ...
Mesquita, D. P.; Leal, C.; Cunha, J. R.; Oehmen, Adrian; Amaral, A. L.; Reis, Maria A. M.; Ferreira, E. C.The present study focuses on predicting the concentration of intracellular storage polymers in enhanced biological phosphorus removal (EBPR) systems. For that purpose, quantitative image analysis techniques were developed for determining the intracellular concentrations of PHA (PHB and PHV) with Nile blue and glycogen with aniline blue staining. Partial least squares (PLS) were used to predict the standard anal...
Novel method to quantify intracellular accumulation of polyphosphate in EBPR sy...
Mesquita, D. P.; Amaral, A. L.; Leal, Cristiano; Carvalheira, Mónica; Cunha, J. R.; Oehmen, Adrian; Reis, Maria A. M.; Ferreira, E. C.A new method for intracellular storage polyphosphate (poly-P)identification and quantification in enhanced biological phosphorus removal (EBPR) systems is proposed based on image analysis. In EBPR systems, 4',6-diamidino-2-phenylindole (DAPI) is usually combined with fluororescent in situ hybridization (FISH) to evaluate the microbial community. The proposed technique is based on an image analysis procedure spe...
Biopolymer monitoring using quantitative image analysis techniques
Carvalheira, Mónica; Mesquita, D. P.; Leal, Cristiano; Oehmen, Adrian; Carvalho, Gilda; Cunha, J. R.; Amaral, A. L.; Ferreira, E. C.; Reis, Maria A. M.Polyhydroxyalkanoates (PHAs) are intracellular biopolymers with many applications, particularly as substitutes of polypropylene and polyethylene, due to their thermoplastic properties and biocompatible nature. Furthermore, glycogen is a polysaccharide of glucose with high importance in the metabolism of microbial communities and polyphosphate is a microbial storage compound that should be recovered in order to ...
Intracellular poly-P assessment by DAPI staining and image analysis
Amaral, A. L.; Mesquita, D. P.; Leal, C.; Carvalheira, Mónica; Oehmen, Adrian; Reis, Maria A. M.; Ferreira, E. C.In wastewater treatment, enhanced biological phosphorus removal (EBPR) is considered a well-established process to remove phosphate (P). EBPR is based on the activity of polyphosphate-accumulating organisms (PAOs) able to take up and store large amounts of P as intracellular (poly-P) granules. However, monitoring poly-P in mixed cultures is usually performed by a laborious and time consuming off-line chemical a...
Desenvolvimento de metodologias de análise de imagem para quantificar PHA, poli...
Amaral, A. L.; Leal, Cristiano S.; Mesquita, D. P.; Cunha, J. R.; Oehmen, Adrian; Reis, Maria A. M.; Ferreira, E. C.O processo de remoção biológica de fósforo, em estações de tratamento de águas residuais, é um processo efetuado por culturas mistas contendo organismos acumuladores de polifosfatos (PAO) e de glicogénio (GAO). No decurso deste processo os microrganismos podem formar inclusões de glicogénio, polihidroxialcanoatos (PHA) e polifosfatos (poli-P). Neste processo, é fulcral monitorizar o metabolismo intracelular par...
Prediction of intracellular storage polymers using quantitative image analysis ...
Mesquita, D. P.; Leal, C.; Cunha, J. R.; Oehmen, Adrian; Amaral, A. L.; Reis, Maria A. M.; Ferreira, E. C.Intracellular polymers, such as polyhydroxyalkanoates (PHA) synthesized by microorganisms for energy and carbon storage, can be commercially used as biodegradable plastics in a wide range of applications. The possibility of obtaining high PHA contents (where the most common monomers found are poly-hydroxybutyrate (PHB) and poly-hydroxyvalerate (PHV)) from inexpensive inocula and raw materials emerges as a promi...
Polyphosphates and poly-β-hydroxybutyrate granules identification through quant...
Mesquita, D. P.; Leal, Cristiano; Oehmen, Adrian; Amaral, A. L.; Reis, Maria A. M.; Ferreira, E. C.Enhanced biological phosphorus removal (EBPR) is a widely implemented technique for having the potential to cheaply and reliably remove phosphate from wastewater treatment processes, than traditional chemical methods. EBPR is performed by operating the system sequentially with anaerobic and aerobic conditions. Several studies were already performed ranging from different strategies for the competition between p...
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