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Random and direct mutagenesis to enhance protein secretion in Ashbya gossypii
Ribeiro, Orquídea; Magalhães, Frederico; Aguiar, Tatiana Quinta; Wiebe, Marilyn G.; Penttilä, Merja; Domingues, LucíliaTo improve the general secretion ability of the biotechnologically relevant fungus Ashbya gossypii, random mutagenesis with ethyl methane sulfonate (EMS) was performed. The selection and screening strategy followed revealed mutants with improved secretion of heterologous Trichoderma reesei endoglucanase I (EGI), native α-amylase and/or native β-glucosidase. One mutant, S436, presented 1.4- to 2-fold increases i...
Characterization of the Ashbya gossypii secreted N-glycome and genomic insights...
Aguiar, Tatiana Quinta; Maaheimo, Hannu; Heiskanen, Annamari; Wiebe, Marilyn G.; Penttilä, Merja; Domingues, LucíliaThe riboflavin producer Ashbya gossypii is a filamentous hemiascomycete, closely related to the yeast Saccharomyces cerevisiae, that has been used as a model organism to study fungal developmental biology. It has also been explored as a host for the expression of recombinant proteins. However, although N-glycosylation plays important roles in protein secretion, morphogenesis, and the development of multicellula...
Nutritional requirements and strain heterogeneity in Ashbya gossypii
Ribeiro, Orquídea; Domingues, Lucília; Penttilä, Merja; Wiebe, Marilyn G.Colony radial growth rates and specific growth rates of three related Ashbya gossypii strains ATCC10895, IMI31268, MUCL29450 and an unrelated strain, CBS109.26, were measured on various carbon and nitrogen sources at pH 4.5 and pH 6.5 to elucidate physiological growth requirements and strain differences. All strains grew on yeast extract or ammonium as nitrogen sources, but not on nitrate. Substantial growth at...
Biotechnological versatility of riboflavin producer Ashbya gossypii
Ribeiro, Orquídea; Wiebe, Marilyn G.; Penttilä, Merja; Domingues, LucíliaEste resumo faz parte de: Book of abstracts of the Meeting of the Institute for Biotechnology and Bioengineering, 2, Braga, Portugal, 2010. A versão completa do livro de atas está disponível em: http://hdl.handle.net/1822/10968
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