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A bi-layer electrospun nanofiltration membrane for plasmid DNA recovery from fe...
Correia, Tiago R.; Antunes, Bernardo P.; Castilho, Pedro H.; Nunes, José C.; Amorim, M. T. Pessoa de; Escobar, I. C.; Correia, I. J.; Morão, A.The demanding ever-increasing quantities of highly purified biomolecules by bio-industries, has triggered the development of new, more efficient, purification techniques. The application of membrane-based technologies has become very attractive in this field, for their high throughput capability, simplicity of operation and scale-up. Herein we report the production of a bi-layer membrane by electrospinning (ES...
Plasmid DNA recovery from fermentation broths by a combined process of micro- a...
Nunes, José C.; Morão, A.; Nunes, Catherine; Amorim, M. T. Pessoa de; Escobar, I. C.; Queiroz, J. A.Microfiltration and ultrafiltration operations were used in tandem to isolate and purify a 6050 bp plasmid DNA (pDNA). To achieve primary isolation of the plasmid from fermentation broths, immediately after cell lysis, a 0.2 μm microfiltration membrane was selected for solid/liquid separation, which was performed in a diafiltration mode, as an alternative to centrifugation. Then to attain plasmid concentration ...
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