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Plasmid DNA recovery from fermentation broths by a combined process of micro- a...
Nunes, José C.; Morão, A.; Nunes, Catherine; Amorim, M. T. Pessoa de; Escobar, I. C.; Queiroz, J. A.Microfiltration and ultrafiltration operations were used in tandem to isolate and purify a 6050 bp plasmid DNA (pDNA). To achieve primary isolation of the plasmid from fermentation broths, immediately after cell lysis, a 0.2 μm microfiltration membrane was selected for solid/liquid separation, which was performed in a diafiltration mode, as an alternative to centrifugation. Then to attain plasmid concentration ...
Data: 2012
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Origem: RepositóriUM - Universidade do Minho
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