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Non-coding RNAs: multi-tasking molecules in the cell
Gomes, Anita Q.; Nolasco, Sofia; Soares, HelenaIn the last years it has become increasingly clear that the mammalian transcriptome is highly complex and includes a large number of small non-coding RNAs (sncRNAs) and long noncoding RNAs (lncRNAs). Here we review the biogenesis pathways of the three classes of sncRNAs, namely short interfering RNAs (siRNAs), microRNAs (miRNAs) and PIWI-interacting RNAs (piRNAs). These ncRNAs have been extensively studied and ...
The expression of tubulin cofactor A (TBCA) is regulated by a noncoding antisen...
Nolasco, Sofia; Bellido, Javier; Gonçalves, João; Zabala, Juan Carlos; Soares, Helena; Tavares, AlexandraAbstract - Recently, long noncoding RNAs have emerged as pivotal molecules for the regulation of coding genes' expression. These molecules might result from antisense transcription of functional genes originating natural antisense transcripts (NATs) or from transcriptional active pseudogenes. TBCA interacts with β-tubulin and is involved in the folding and dimerization of new tubulin heterodimers, the building ...
Besnoitia besnoiti protein disulfide isomerase (BbPDI): molecular characterizat...
Marcelino, Eduardo; Martins, Tiago M.; Morais, Joana B.; Nolasco, Sofia; Cortes, Helder; Hemphill, Andrew; Leitão, Alexandre; Novo, CarlosBesnoitia besnoiti is an apicomplexan parasite responsible for bovine besnoitiosis, a disease with a high prevalence in tropical and subtropical regions and re-emerging in Europe. Despite the great economical losses associated with besnoitiosis, this disease has been underestimated and poorly studied, and neither an effective therapy nor an efficacious vaccine is available. Protein disulfide isomerase (PDI) is ...
TBCCD1, a new centrosomal protein, is required for centrosome and Golgi apparat...
Gonçalves, João; Nolasco, Sofia; Nascimento, Rute; Lopez Fanarraga, Mónica; Zabala, Juan Carlos; Soares, HelenaIn animal cells the centrosome is positioned at the cell centre in close association with the nucleus. The mechanisms responsible for this are not completely understood. Here, we report the first characterization of human TBCC-domain containing 1 (TBCCD1), a protein related to tubulin cofactor C. TBCCD1 localizes at the centrosome and at the spindle midzone, midbody and basal bodies of primary and motile cilia....
Tubulin cofactor A gene silencing in mammalian cells induces changes in microtu...
Nolasco, Sofia; Bellido, Javier; Gonçalves, João; Zabala, Juan Carlos; Soares, HelenaMicrotubules are polymers of alpha/beta-tubulin participating in essential cell functions. A multistep process involving distinct molecular chaperones and cofactors produces new tubulin heterodimers competent to polymerise. In vitro cofactor A (TBCA) interacts with beta-tubulin in a quasi-native state behaving as a molecular chaperone. We have used siRNA to silence TBCA expression in HeLa and MCF-7 mammalian ce...
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